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Correlation between pediatric donor characteristics and cell compositions in mixture allografts of combined G-CSF-mobilized PBSCs and bone marrow allografts

Bone Marrow Transplantation volume 53, pages 108–110 (2018)Cite this article

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Allogeneic stem cell transplantation (allo-SCT), including HLA-identical sibling donor and alternative donor transplant, provides a potentially curable method for patients with hematological malignancies.1 Rapid hematopoietic reconstitution and immune recovery are key steps to a successful transplantation. In allo-SCT settings, several studies have demonstrated the effects of cell compositions in allografts with clinical outcomes.2, 3, 4, 5, 6 Previous studies show that donor characteristics, such as age, gender and body mass index, and donors’ leukocyte count, contribute to the hematopoietic and immune cell yield, although controversy remains.7, 8, 9, 10 However, nearly all of these studies focus on the correlation of adult demographic features with cell compositions in the allografts. Few studies have reported the impacts of pediatric donor characteristics on cell compositions in mixture allografts.4

We prospectively analyzed 104 healthy pediatric donors who underwent granulocyte-CSF (G-CSF)-mobilized bone marrow (G-BM) and PBSC harvests (G-PB) between January 2014 and June 2016 to investigate the correlation between donor characteristics and the doses of hematopoietic and immune compositions in mixture allografts of G-PB and G-BM. The mobilization, bone marrow and peripheral blood allograft collection, flow cytometry evaluation of graft composition and statistical analysis were conducted according to our previous study.9, 10 The characteristics and blood cell counts of 104 healthy donors are showed in Table 1. The medians and ranges of cellular compositions in mixture allografts, including CD34+ cells, CD3+ T cells, CD3+CD4+ T cells, CD3+CD8+ T cells, CD3+CD4−CD8− T cells and monocytes are 3.79 (0.78–13.67) × 106/kg, 234.73 (92.47–579.12) × 106/kg, 107.61 (40.86–258.52) × 106/kg, 102.34 (21.18–305.74) × 106/kg, 19.07 (5.1–80.34) × 106/kg and 170.09 (46.42–688.75) × 106/kg, respectively.

Table 1 Donor characteristics
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Acknowledgements

This work was supported (in part) by the National Natural Science Foundation of China (Grant No. 81470342). We thank the faculty members who collected samples and analyzed flow cytometry data.

Author contributions

Y-JC designed the study and collected the data; Y-JC, L-QC and LL analyzed the data and wrote the typescript; and all authors contributed to interpretation of the data, preparing the typescript and approved the final version.

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  1. L-Q Cao and L Liu: These authors contributed equally to this work.

Authors and Affiliations

  1. Peking University People’s Hospital, Peking University Institute of Hematology, Beijing, China

    L-Q Cao, L Liu, L-P Xu, X-H Zhang, Y Wang, Q-Z Fan, Y-R Liu, K-Y Liu, X-J Huang & Y-J Chang

  2. Peking-Tsinghua Center for Life Sciences, Beijing, China

    X-J Huang

  3. Collaborative Innovation Center of Hematology, Peking University, Beijing, China

    Y-J Chang

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  1. L-Q Cao
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Cao, LQ., Liu, L., Xu, LP. et al. Correlation between pediatric donor characteristics and cell compositions in mixture allografts of combined G-CSF-mobilized PBSCs and bone marrow allografts. Bone Marrow Transplant 53, 108–110 (2018). https://doi.org/10.1038/bmt.2017.224

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