Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter to the Editor
  • Published:

Infused neutrophil dose and haematopoietic recovery in patients undergoing autologous transplantation

Bone Marrow Transplantation volume 49, page 725 (2014)Cite this article

Subjects

Autologous haematopoietic SCT (HSCT) is now a standard treatment for a variety of relapsed or refractory haematological malignancies. Engraftment following HSCT is dependent upon the number and viability of stem cells in the graft.1, 2 The degree of neutrophil contamination in haematopoietic progenitor cell–apheresis (HPC-A) products has been inversely correlated to post-thaw nucleated cell recovery.3 However, the correlation between haematopoietic recovery in autologous transplant recipients and the degree of neutrophil contamination in HPC-A has not been analysed. Therefore, we assessed the data from 446 consecutive patients who underwent autologous transplantation at our centre using HPC-A between 2001 and 2012. Correlation between the infused dose of neutrophils and the times to neutrophil and platelet engraftment was analysed using Pearson’s correlation analysis. Other factors including age, gender, disease, infused CD34+ cell dose and disease status at the time of transplantation were also studied to assess potential impacts on engraftment.

Increasing neutrophil contamination of HPC-A was strongly associated with slower neutrophil recovery (R2=0.035, P<0.005). By univariate analysis, infused neutrophil dose together with disease, and disease status (CR or not) at the time of transplantation were the factors that had an impact on neutrophil engraftment. Infused neutrophils ⩾4 × 108/kg vs <4 × 108/kg resulted in longer times to neutrophil engraftment—12 (9–29) days vs 11 (8–28) days (P<0.005). There was a small but significant delay in neutrophil recovery associated with disease: transplantation for leukaemia or multiple myeloma resulted in a longer time to neutrophil engraftment when compared with a diagnosis of lymphoma (P=0.003). Factors affecting platelet engraftment were gender, disease, infused and post-thaw viable CD34+ cell dose. Age at the time of transplantation did not have a significant impact on engraftment on univariate analysis.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Allan DS, Keeney M, Howson-Jan K, Popma J, Weir K, Bhatia M et al. Number of viable CD34+ cells reinfused predicts engraftment in autologous hematopoietic stem cell transplantation. Bone Marrow Transplant 2002; 29: 967–972.

    Article  CAS  Google Scholar 

  2. Weaver CH, Hazelton B, Birch R, Palmer P, Allen C, Schwartzberg L et al. An analysis if engraftment kinetics as a function of the CD34 content of peripheral blood progenitor cell collections in 692 patients after the administration of myeloablative chemotherapy. Blood 1995; 86: 3961–3969.

    CAS  Google Scholar 

  3. Foïs E, Desmartin M, Benhamida S, Xavier F, Vanneaux V, Rea D et al. Recovery, viability and clinical toxicity of thawed and washed haematopoietic progenitor cells: analysis of 952 autologous peripheral blood stem cell transplantations. Bone Marrow Transplant 2007; 40: 831–835.

    Article  Google Scholar 

  4. Rowley SD . Techniques of bone marrow and stem cells cryopreservation and storage. In: Sacher RA, Aubuchon JP (eds).. Marrow Transplantation: Practice and Technical Aspects of Stem Cells Reconstitution. American Association of Blood Banks: Bethesda, MD, USA, 1992 pp 105–127.

    Google Scholar 

  5. Jansen J, Thompson JM, Dugan MJ, Nolan P, Wiemann MC, Birhiray R et al. Peripheral blood progenitor cell transplantation. Ther Apher 2002; 6: 5–14.

    Article  Google Scholar 

  6. Hicks C, Wong R, Manoharan A, Kwan YL . Viable CD34+/CD133+ blood progenitor cell dose as a predictor of haematopoietic engraftment in multiple myeloma patients undergoing autologous peripheral blood stem cell transplantation. Ann Hematol 2007; 86: 591–598.

    Article  CAS  Google Scholar 

  7. Lefrère F, Delarue R, Somme D, Levy V, Damaj G, Tu A et al. High-dose CD34+ cells are not clinically relevant in reducing cytopenia and blood component consumption following myeloablative therapy and peripheral blood progenitor cell transplantation as compared with standard dose. Transfusion 2002; 42: 443–450.

    Article  Google Scholar 

  8. Hess DA, Wirthlin L, Craft TP, Herrbrich PE, Hohm SA, Lahey R et al. Selection based on CD133 and high aldehyde dehydrogenase activity isolates long-term reconstituting human hematopoietic stem cells. Blood 2006; 107: 2162–2169.

    Article  CAS  Google Scholar 

  9. Lioznov MV, Freiberger P, Kröger N, Zander AR, Fehse B . Aldehyde dehydrogenase activity as a marker for the quality of hematopoietic stem cell transplants. Bone Marrow Transplant 2005; 35: 909–914.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Haematology and Transfusion Medicine, Royal North Shore Hospital, Sydney, NSW, Australia

    L Bai, W Xia, K Wong, C Reid, C Ward & M Greenwood

  2. Cellular Therapeutic Laboratory, Northern Blood Research Centre, Kolling Research Institute, Sydney, NSW, Australia

    L Bai & W Xia

Authors
  1. L Bai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K Wong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C Reid
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C Ward
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M Greenwood
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to L Bai.

Ethics declarations

Competing interests

The authors declare no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bai, L., Xia, W., Wong, K. et al. Infused neutrophil dose and haematopoietic recovery in patients undergoing autologous transplantation. Bone Marrow Transplant 49, 725 (2014). https://doi.org/10.1038/bmt.2014.14

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/bmt.2014.14

Search

Advanced search

Quick links