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Graft Source

Cord blood transplantation is associated with rapid B-cell neogenesis compared with BM transplantation

Bone Marrow Transplantation volume 49, pages 1155–1161 (2014)Cite this article

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Abstract

Hematopoietic cell transplantation (HCT) is used for treatment of hematopoietic diseases. Assessment of T- and B-cell reconstitution after HCT is crucial because poor immune recovery has a major effect on the clinical course. In this study, we retrospectively analyzed T-cell receptor excision circles (TRECs) as well as signal and coding joint kappa-deleting recombination excision circles (sjKRECs and cjKRECs, respectively) as markers of newly produced lymphocytes in 133 patients (56 primary immunodeficient and 77 malignant cases, median (range): 12 (0–62) years old). We analyzed the kinetics of TREC and KREC recovery and determined the factors that contributed to better immune recovery. KRECs became positive earlier than TRECs and increased thereafter. Younger recipient age had a favorable effect on recovery of sjKRECs and cjKRECs. Compared with BM and peripheral blood, our data suggested that cord blood (CB) provided rapid B-cell recovery. CB also provided better B-cell neogenesis in adult HCT recipients. Chronic GVHD was associated with low TRECs, but not increased sjKRECs/cjKRECs. Finally, positive sjKRECs 1 month after HCT were associated with fewer infectious episodes. Monitoring of TRECs and KRECs may serve as a useful tool for assessment of immune reconstitution post HCT.

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Acknowledgements

This work was in part supported by Health and Labour Sciences Research Grants for Intractable diseases (H23-003 and H24-008 to TM, and H24-013 to KI).

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Authors and Affiliations

  1. Department of Pediatrics and Developmental Biology, Tokyo Medical and Dental University Graduate School of Medical and Dental Sciences, Tokyo, Japan

    K Nakatani, M Shigeno, N Mitsuiki, S Mizutani & T Morio

  2. Department of Pediatrics, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan

    K Imai, M Tezuka, T Okawa, T Isoda, D Tomizawa, M Takagi, M Nagasawa, S Mizutani & T Morio

  3. Department of Preventive Medicine and Public Health, National Defense Medical College, Saitama, Japan

    H Sato

  4. Department of Blood Transfusion, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan

    M Kajiwara

  5. Department of Hematology, Tokyo Medical and Dental University Medical Hospital, Tokyo, Japan

    M Yamamoto, A Arai & O Miura

  6. Department of Pediatrics, National Defense Medical College, Saitama, Japan

    C Kamae, N Nakagawa, K Honma & S Nonoyama

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  1. K Nakatani
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  2. K Imai
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Correspondence to T Morio.

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Nakatani, K., Imai, K., Shigeno, M. et al. Cord blood transplantation is associated with rapid B-cell neogenesis compared with BM transplantation. Bone Marrow Transplant 49, 1155–1161 (2014). https://doi.org/10.1038/bmt.2014.123

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  • DOI: https://doi.org/10.1038/bmt.2014.123

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