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Transplant Technology

Transportation and cryopreservation may impair haematopoietic stem cell function and engraftment of allogeneic PBSCs, but not BM

Bone Marrow Transplantation volume 42, pages 121–128 (2008)Cite this article

Abstract

Recent data suggest that the practice of using frozen allogeneic grafts is becoming increasingly common among transplant centres. Therefore, we retrospectively analysed 31 frozen allogeneic PBSC and 8 BM grafts by flow cytometry with regard to their CD34+ content, membrane integrity (7-AAD) and stem cell-specific enzyme activity (aldehyde dehydrogenase, ALDH) in relation to individual transplantation results. Membrane integrity of CD34+ cells was significantly impaired in cryopreserved PBSC but not in BM compared to unfrozen allografts. In 9 out of 31 frozen PBSC (but none of the BM) grafts numbers of SSCloALDHbr cells per kg body weight (BW) were significantly reduced while in the same grafts the numbers of CD34+ cells per kg BW were close to normal. Overall, 9 out of 33 patients (27%) who received unrelated PBSC allografts cryopreserved after transportation did not achieve engraftment. For comparison, primary graft failure was observed in our centre in only 7 out of 493 recipients (1.4%) of fresh allogeneic PBSC grafts. Moreover, we did not see any graft failure in patients receiving frozen/thawed BM or autologous PBSC transplants. We, therefore, conclude that PBSC grafts become much more sensitive to cryopreservation after transport and/or storage. Importantly, the engraftment potential of frozen HSC grafts may reliably be predicted by measuring ALDH activity.

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Acknowledgements

We thank P Freiberger, U Fritzsche-Friedland, E Merle and M Reckhaus for the excellent technical assistance, and G Amtsfeld and J Hagelberg for their help with clinical data.

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  1. B Fehse

    Present address: 3Current address: Experimental Paediatric Oncology and Haematology, Paediatric Clinic III, University Hospital of the Johann Wolfgang Goethe-University, Frankfurt am Main, Germany.,

Authors and Affiliations

  1. Clinic for Stem Cell Transplantation, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany

    M Lioznov, C Dellbrügger, B Fehse, N Kröger & A R Zander

  2. Department for Transfusion Medicine, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany

    A Sputtek

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  1. M Lioznov
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  2. C Dellbrügger
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Correspondence to A R Zander.

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Lioznov, M., Dellbrügger, C., Sputtek, A. et al. Transportation and cryopreservation may impair haematopoietic stem cell function and engraftment of allogeneic PBSCs, but not BM. Bone Marrow Transplant 42, 121–128 (2008). https://doi.org/10.1038/bmt.2008.93

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