Abstract
The indirect immunofluorescence technique demonstrated that human alpha fetoprotein (AFP) was present in a focal pattern in the cytoplasm of malignant liver cells of patients whose sera contained AFP. A few fibroblastlike cells in tissue culture of liver biopsies from patients with hepato-cellular carcinoma and AFP in their sera also had the protein. The intracellular localisation of human AFP was confirmed by centrifugation of washed, homogenised and ultra-sonically disrupted neonatal liver cells. Examination of livers of fetuses and neonates showed that AFP was present predominantly in the periportal parenchymal cells.
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Smith, J., Francis, T., Edington, G. et al. Immunofluorescent Localisation of Human Alpha Fetoprotein in Fetal and Neonatal Livers and Cultured Cells from Hepatocellular Carcinoma. Br J Cancer 25, 343–349 (1971). https://doi.org/10.1038/bjc.1971.44
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DOI: https://doi.org/10.1038/bjc.1971.44
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