Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Novel KCNQ2 channel activators discovered using fluorescence-based and automated patch-clamp-based high-throughput screening techniques

Abstract

Aim:

To establish an improved, high-throughput screening techniques for identifying novel KCNQ2 channel activators.

Methods:

KCNQ2 channels were stably expressed in CHO cells (KCNQ2 cells). Thallium flux assay was used for primary screening, and 384-well automated patch-clamp IonWorks Barracuda was used for hit validation. Two validated activators were characterized using a conventional patch-clamp recording technique.

Results:

From a collection of 80 000 compounds, the primary screening revealed a total of 565 compounds that potentiated the fluorescence signals in thallium flux assay by more than 150%. When the 565 hits were examined in IonWorks Barracuda, 38 compounds significantly enhanced the outward currents recorded in KCNQ2 cells, and were confirmed as KCNQ2 activators. In the conventional patch-clamp recordings, two validated activators ZG1732 and ZG2083 enhanced KCNQ2 currents with EC50 values of 1.04±0.18 μmol/L and 1.37±0.06 μmol/L, respectively.

Conclusion:

The combination of thallium flux assay and IonWorks Barracuda assay is an efficient high-throughput screening (HTS) route for discovering KCNQ2 activators.

Introduction

With more than 40 members, voltage-gated potassium (Kv) channels are a major drug target group that is essential for treating a variety of human diseases. The neuronal KCNQ2 potassium channel (Kv7.2) is a low-threshold Kv channel that is expressed in both the central and peripheral nervous systems1,2,3. As an essential player that mediates neuronal muscarinic (M) currents and controls neuronal excitability, activating a KCNQ2 channel or heterogeneous KCNQ2 complexes will inhibit action potential initiation and, thus, dampen neuronal excitability2. In contrast, inhibiting the channel causes hyperexcitability4,5,6. Mutations in human KCNQ2 genes that reduce or eliminate channel activity cause a single type of familial convulsion7,8. Activation of the KCNQ2 channel by synthetic compounds may dampen membrane excitability and, therefore, may be a strategy for treating hyperexcitability conditions, such as epilepsy and neuropathic pain3. In 2011, retigabine (RTG) became the first approved KCNQ activator to treat human epilepsy9. With the exception of retigabine, few novel KCNQ activators have been reported in the past few decades8,10,11,12,13,14,15,16. Identifying novel activators is beneficial for understanding channel activity mechanisms and developing new therapeutics. For example, one study used ztz240 as a chemical probe to demonstrate that the KCNQ2 channel voltage-sensing domains are novel anti-epilepsy targets17. Benzbromarone (BBR) is a KCNQ activator that primarily acts upon peripheral KCNQ channels. The unique tissue distribution of BBR allowed scientists to resolve a long-standing debate concerning the central or peripheral mediation of analgesic activity of KCNQ activators. This provided a new strategy for developing more effective pain therapies10.

To discover novel small molecule activators for KCNQ2 channels from large compound libraries, various high-throughput screening (HTS) techniques have been developed. Fluorescence-based assays provide robust and homogeneous cell population measurements and have been widely used for both cation and anion-permeable channels. K+ channels are permeable to Tl+18. The Tl+ indicator FluxOR has been used in conjunction with a fluorescent reader for the high-throughput detection of intracellular Tl+ concentration changes. The thallium flux assay has been used to identify K+ channel activators in multiple large-scale screening experiments19,20,21. Electrophysiological recording directly provides ionic currents and is considered to be a gold standard for studying ion channels. Nevertheless, due to their low throughput, conventional patch-clamp experiments are not appropriate for large-scale screening. Over the past few decades, many automated electrophysiology platforms with highly improved throughput have been developed. Therefore, directly measuring ionic currents in HTS is a reality22,23,24. The IonWorks Barracuda is a 384-well format automated patch that relies on a perforated patch-clamp technique. Compared with earlier generations of this instrument, the IonWorks Barracuda provides better throughput and data consistency; however, the feasibility of using the IonWorks Barracuda in a KCNQ2 study has not been reported. Herein, we screened a collection of 80 000 compounds using the thallium flux assay as the primary screen and then used the IonWorks Barracuda for a secondary validation. Two validated hits were further characterized using a conventional patch clamp, which revealed an EC50 of 1–2 μmol/L. Our study provides an efficient HTS means for identifying novel KCNQ2 activators (Figure 1).

Figure 1
figure 1

Flow-chart of the high-throughput screening route for identifying KCNQ2 activators. The fluorescence-based thallium flux assay was used for the primary screen, followed by the confirmatory 384-well IonWorks Barracuda for hit validation. After the hit validation, a conventional patch-clamp experiment was used for probe characterization.

PowerPoint slide

Materials and methods

Thallium flux assay

Chinese hamster ovary (CHO) cells that stably expressed KCNQ2 channels were routinely cultured in F-12 medium supplemented with 10% FBS and 250 μg/mL Hygromycin B. KCNQ2 cells were seeded into a 384-well black clear bottom plate at a concentration of 8000 cells/well by a multidrop dispenser, and the plate was incubated overnight at 37 °C in a 5% CO2 incubator. The next day, after manual removal of the medium, 25 μL/well dye-loading buffer (1000× FluxOR reagent stock, 100× PowerLoad, 1× Hanks' balanced salt solution, 20 mmol/L HEPES, and 2.5 mmol/L Probenecid, pH 7.40) was added into the cell plate, and the plate was then incubated in the dark at room temperature for 90 min. Once the dye-loading buffer was manually removed, the cell plates were then supplemented with 20 μL/well assay buffer (1× Hank's balanced salt solution, 20 mmol/L HEPES, and 2.5 mmol/L Probenecid, pH 7.40). Additionally, 4 μL/well compound buffer was added into the plate, and the plate was then incubated at room temperature in the dark. After the tested compounds were incubated for 30 min, cell plates were loaded onto an FDSS (Hamamatsu Photonics). After 10 s of recording, 6 μL/well of stimulus buffer was added. The plates were read every second for 180 s. The stimulus buffer contained 25 mmol/L K+ and 25 mmol/L Tl+. The FluxOR thallium assay protocol that has been described is identical to the manufacturer's protocol. The fluorescence potentiation (Fluo Potenti %=[(RtestRcontrol)/(RcontrolRbuffer)×100] was calculated for each well using the 35 s fluorescence ratio. To evaluate the quality of the thallium flux data, the Z-factor was calculated using the following equation: Z=1-3(SDc+SDn)/|C-N|, where SDc and SDn are the standard deviation of the control group (c) and the group in the presence of positive control group (n), respectively. C and N are the mean values of the control and positive control groups, respectively.

Automated patch-clamp recording

KCNQ2 cells were dissociated from T175 flasks by aspirating off the culture medium, rinsing the cells twice with phosphate buffered saline solution (PBS, Invitrogen) and adding 3 mL trypsin-EDTA per flask. The cells were incubated for 3 min in a 37 °C incubator. The flasks were added to 7 mL of cell culture medium, and the cells were gently triturated by pipetting up and down two to three times. Cells were pelleted at 800 rounds per minute for 3 min. The supernatant was discarded and the cell pellet was resuspended in 5 mL of external solution at a density of 1.5–2.0 million cells per mL. The 384-well automated patch clamp IonWorks Barracuda (Molecular Devices) in the population patch clamp (PPC) mode was used for hit validation. The cells were voltage clamped at a holding potential of −100 mV. The KCNQ2 current was activated by depolarizing to −10 mV for 10 s, after which the voltage was taken back to −120 mV for 10 s to observe the deactivating tail current. The maximum amount of outward current size was used to determine KCNQ2 current amplitude.

Conventional patch-clamp recording

Standard whole-cell recording was used to record the current of KCNQ2 channels. Pipettes were pulled from borosilicate glass capillaries (TW150-4, World Precision Instruments, Sarasota, FL, USA). When filled with the intracellular solution, the pipettes had a 3–5 MΩ resistance. During the recording, the extracellular solution was constantly perfused by a BPS perfusion system (ALA Scientific Instruments). The intracellular solution contained (in mmol/L): 140 KCl, 1.75 MgCl2, 10 EGTA, 10 HEPES and 5 Na2ATP (pH=7.2); the extracellular solution contained (in mmol/L): 145 NaCl, 3 KCl, 2 CaCl2, 1 MgCl2, 10 HEPES and 10 glucose (pH=7.40). Both currents and voltages were recorded using an Axopatch-200B amplifier, filtered at 2 kHz, and digitized using a DigiData 1440A with pClamp 10.2 software (Molecular Devices). Series resistance compensation was also used and set to 60%–80%. Patch clamp data were processed using Clampfit 10.2 (Molecular Devices, Sunnyvale, CA, USA) and then analyzed in GraphPad Prism 5 (GraphPad Software). Voltage-dependent activation curves were fitted using the Boltzmann equation, G=Gmin+(GmaxGmin)/(1+exp(VV1/2)/S), where Gmax is the maximum conductance, Gmin is the minimum conductance, V1/2 is the voltage for reaching 50% of maximum conductance, and S is the slope factor. Dose–response curves were fitted with the Hill equation, E=Emax/(1+(EC50/C)P), where EC50 is the drug concentration that produced half of the maximum response, and P is the Hill coefficient.

Statistical analysis

The data are presented as the mean±SEM. The significance was estimated using paired two-tailed Student's t-tests. An effect was considered significant if P<0.05.

Results

Primary screening using the thallium flux assay

To identify compounds with potentiation effects on KCNQ2 channels, we generated a KCNQ2-stable cell line. After a series of optimization experiments for several important screening parameters, which have been described previously, a standard procedure for the thallium flux assay was developed and used to screen an in-house collection of over 80 000 compounds at a 10 μmol/L final concentration (Figure 2). The positive control was ztz240, which is a KCNQ2 channel activator that was previously identified to significantly increase the fluorescence signal by approximately 150% at 10 μmol/L. Of the 80 000 screened compounds, 565 hits were discovered that exhibited a larger potentiation activity than ztz240, as exemplified by ZG1732. The Z factors ranged from 0.51 to 0.89, which are consistent with the assay quality requirement of being greater than 0.5.

Figure 2
figure 2

Results of thallium flux assay. (A and B) Representative fluorescence traces in the absence or presence of 10 μmol/L ztz240 (A) or 10 μmol/L ZG1732 (B). (C) Z factors of the thallium flux assay. (D) Distribution of identified hits in a total of 80 000 compounds in the thallium flux assay. The x-axis represents the potentiation activity of the hits on the KCNQ2-mediated fluorescence signal (see the Methods for the calculation method).

PowerPoint slide

Hit validation using an automated patch-clamp experiment

A 384-well automated patch-clamp instrument, the IonWorks Barracuda, was used to validate the hits. The stabilities of the KCNQ2 currents were evaluated first. The cells were held at −100 mV and stimulated by depolarization to −10 mV every 20 s. Prior to each depolarization event, a 20-ms pre-pulse that polarizes the membrane potential to −90 mV (a “sweep”) was delivered for leak subtraction. Under these conditions, the outward currents of the KCNQ2 channels remained relatively stable for 5 min. We then examined the KCNQ2 current responses to 10 μmol/L ztz240. As shown in Figure 3, the drug was applied after the 3rd sweep. The increased KCNQ2 currents reached a steady state by the 5th sweep, and a slight run-down was observed at the 7th sweep. Therefore, the currents measured at the 6th sweep were used to determine the potentiation activity of the tested hits. Of the 565 hits that were discovered in the primary screen, 38 were confirmed as KCNQ2 channel activators (Figure 3C and 3D, and Supplementary Table S1). Most of the validated activators displayed comparable potentiation activity to ztz240, which is exemplified by ZG1732.

Figure 3
figure 3

Results of IonWorks Barracuda assay. (A) Stability of KCNQ2 currents that were recorded using the IonWorks Barracuda instrument. ztz240 (10 μmol/L) was applied at the 3rd sweep (a). The increased KCNQ2 current was measured before the 'run-down' appeared (the 6th sweep (b). (B) Representative KCNQ2 currents before and after exposure to 10 μmol/L ztz240. (C) Potentiation of the validated hits on the KCNQ2 currents. (D) Representative KCNQ2 currents before and after exposure to 10 μmol/L ZG1732 [red point in (C)].

PowerPoint slide

Characterization of activators using conventional patch clamp

Among the 38 validated activators, ZG1732 and ZG2083, which belong to different scaffolds, were selected for further characterization using a conventional whole-cell patch-clamp, the gold standard for studying ion channels. Similar to the KCNQ2 activators that were identified previously, including retigabine and ztz240, both ZG1732 and ZG2083 increased the outward KCNQ2 currents and shifted the activation curve to the left (Figure 4). The EC50 values of ZG1732 and ZG2083 were 1.04±0.18 μmol/L and 1.37±0.06 μmol/L, respectively. The micromolar EC50 values are comparable to the hits that were identified in a previous HTS25.

Figure 4
figure 4

Potentiation effects of the hits ZG1732 and ZG2083 on KCNQ2 channels using a conventional patch-clamp. (A) Representative currents of KCNQ2 channels in the absence or presence of indicated hits. To elicit the KCNQ2 currents, depolarization steps that ranged from −90 mV to +60 mV in 10 mV increments were applied from the holding potential of −100 mV. (B and C) ZG1732 and ZG2083 left-shifted the conductance-voltage (G-V) curve of KCNQ2 channels. For ZG1732, ΔV1/2=−19.88±0.12 mV (n=5); for ZG2083, ΔV1/2=−27.79±0.73 mV (n=4). (D) Potentiation activity of 10 μmol/L ZG1732 (I/I0=2.54±0.14, n=4) and 10 μmol/L ZG2083 (I/I0=1.88±0.02, n=7) on the KCNQ2 current amplitude measured at −10 mV. (E) Dose-response curves of ZG1732 and ZG2083 (n=5). Mean±SEM. cP<0.01.

PowerPoint slide

Discussion

In the current study, the thallium flux assay was used as the primary screening method, and the 384-well IonWorks Barracuda instrument was used for the secondary validation. Currently, the thallium flux assay remains the prevalent HTS method for identifying potassium channel modulators, including KCNQ channels19,25. The thallium assay can be used to identify both activators and inhibitors of KCNQ channels. Typically, the hits identified in a primary screen require further chemical modification to improve potency and druggability. For example, a potent KCNQ1 activator was developed based upon an initial hit that was identified in an HTS of the Molecular Libraries Probe Centers Network (MLPCN) library using the thallium assay26. In another HTS by the same group, a potent activator of KCNQ2 channels was designed and synthesized based upon the identified hits25; however, due to the off-target effects and autofluorescence of the compounds, a high false positive hit rate is a major issue for this indirect fluorescence assay. In a screen for KCNQ4 activators, the false positive rate was greater than 93% (623/666) in the primary screening stage21. Consistent with this previous study, the false positive rate for our thallium assay was also 93% (527/565). To reduce the false positive rate, a secondary confirmation is often required with duplicates for each compound and counter screening for the parental cells that do not express the KCNQ channels investigated21. A conventional patch-clamp experiment is the gold standard for studying ion channels and provides a large quantity of information as well as high resolution; however, due to intrinsic limitations, particularly due to its low throughput, conventional patch-clamp is not able to satisfy the need of high-throughput drug discovery27. In response to the demand for high-throughput experiments, many automated patch-clamp systems have been developed throughout the last 30 years28. These automated patch-clamp systems can be divided into automated conventional electrophysiology platforms that use glass pipettes and automated planar-array electrophysiology platforms. Nevertheless, only the latter have overcome the throughput limitations and were first used for screening in 200329. The IonWorks Barracuda is a 384-well planar-array platform that can simultaneously record 384 currents. As a high-throughput patch-clamp instrument that is specifically designed for high-throughput drug discovery, the utility of IonWorks Barracuda must be tested and validated for various ion channels. In the current study, the IonWorks Barracuda can stably record KCNQ2 currents for up to 5 min; thus, it is feasible to evaluate KCNQ2 activators that typically reach steady potentiation within a few minutes. The validated activators were further characterized using conventional patch clamp studies, which confirmed the IonWorks Barracuda results. In summary, our study established an efficient HTS means for identifying KCNQ2 activators, which can be referred to or is applicable for other voltage-gated potassium channels.

Author contribution

Zhao-bing GAO and Fa-Jun NAN designed the project; Jin-feng YUE, Guan-hua QIAO, Ni Liu, and Xue-qin CHEN performed the experiments; all authors analyzed the data; Jin-feng YUE and Zhao-bing GAO wrote the manuscript.

References

  1. Brown DA, Adams PR . Muscarinic suppression of a novel voltage-sensitive K+ current in a vertebrate neurone. Nature 1980; 283 673–6.

    CAS  PubMed  Google Scholar 

  2. Robbins J . KCNQ potassium channels: physiology, pathophysiology, and pharmacology. Pharmacol Ther 2001; 90 1–19.

    CAS  Article  PubMed  Google Scholar 

  3. Xiong Q, Gao Z, Wang W, Li M . Activation of Kv7 (KCNQ) voltage-gated potassium channels by synthetic compounds. Trends Pharmacol Sci 2008; 29 99–107.

    CAS  Article  PubMed  Google Scholar 

  4. Brown DA, Passmore GM . Neural KCNQ (Kv7) channels. Br J Pharmacol 2009; 156 1185–95.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  5. Piccinin S, Randall AD, Brown JT . KCNQ/Kv7 channel regulation of hippocampal gamma-frequency firing in the absence of synaptic transmission. J Neurophysiol 2006; 95 3105–12.

    CAS  Article  PubMed  Google Scholar 

  6. Yue C, Yaari Y . KCNQ/M channels control spike afterdepolarization and burst generation in hippocampal neurons. J Neurosci 2004; 24 4614–24.

    CAS  Article  PubMed  Google Scholar 

  7. Charlier C, Singh NA, Ryan SG, Lewis TB, Reus BE, Leach RJ, et al. A pore mutation in a novel KQT-like potassium channel gene in an idiopathic epilepsy family Nat Genet 1998; 18: 53–5.

    CAS  PubMed  Google Scholar 

  8. Singh NA, Charlier C, Stauffer D, DuPont BR, Leach RJ, Melis R, et al. A novel potassium channel gene, KCNQ2, is mutated in an inherited epilepsy of newborns. Nat Genet 1998; 18: 25–9.

    CAS  Article  PubMed  Google Scholar 

  9. Tatulian L, Delmas P, Abogadie FC, Brown DA . Activation of expressed KCNQ potassium currents and native neuronal M-type potassium currents by the anti-convulsant drug retigabine. J Neurosci 2001; 21 5535–45.

    CAS  Article  PubMed  Google Scholar 

  10. Zheng Y, Xu H, Zhan L, Zhou X, Chen X, Gao Z . Activation of peripheral KCNQ channels relieves gout pain. Pain 2015; 156 1025–35.

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Bentzen BH, Schmitt N, Calloe K, Brown W, Grunnet M, Olesen SP . The acrylamide (S)-1 differentially affects Kv7 (KCNQ) potassium channels. Neuropharmacology 2006; 51 1068–77.

    CAS  Article  PubMed  Google Scholar 

  12. Blom SM, Schmitt N, Jensen HS . The acrylamide (S)-2 as a positive and negative modulator of Kv7 channels expressed in Xenopus laevis oocytes. PLoS One 2009; 4 e8251.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Fritch PC, McNaughton-Smith G, Amato GS, Burns JF, Eargle CW, Roeloffs R, et al. Novel KCNQ2/Q3 agonists as potential therapeutics for epilepsy and neuropathic pain. J Med Chem 2011; 53: 887–96.

    Article  Google Scholar 

  14. Jia C, Qi J, Zhang F, Mi Y, Zhang X, Chen X, et al. Activation of KCNQ2/3 potassium channels by novel pyrazolo[1,5-a]pyrimidin-7(4H)-one derivatives. Pharmacology 2011; 87: 297–310.

    CAS  Article  PubMed  Google Scholar 

  15. Peretz A, Pell L, Gofman Y, Haitin Y, Shamgar L, Patrich E, et al. Targeting the voltage sensor of Kv7.2 voltage-gated K+ channels with a new gating-modifier. Proc Natl Acad Sci U S A 2010; 107 15637–42.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  16. Wickenden AD, Krajewski JL, London B, Wagoner PK, Wilson WA, Clark S, et al. N-(6-chloro-pyridin-3-yl)-3,4-difluoro-benzamide (ICA-27243): a novel, selective KCNQ2/Q3 potassium channel activator. Mol Pharmacol 2008; 73: 977–86.

    CAS  Article  PubMed  Google Scholar 

  17. Li P, Chen Z, Xu H, Sun H, Li H, Liu H, et al. The gating charge pathway of an epilepsy-associated potassium channel accommodates chemical ligands. Cell Res 2013; 23: 1106–18.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  18. Wagoner PK, Oxford GS . Cation permeation through the voltage-dependent potassium channel in the squid axon. Characteristics and mechanisms. J Gen Physiol 1987; 90: 261–90.

    CAS  Article  PubMed  Google Scholar 

  19. Raphemot R, Kadakia RJ, Olsen ML, Banerjee S, Days E, Smith SS, et al. Development and validation of fluorescence-based and automated patch clamp-based functional assays for the inward rectifier potassium channel Kir4.1. Assay Drug Dev Technol 2013; 11: 532–43.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  20. Yu H, Xu K, Zou B, Wu M, McManus OB, Le Engers J, et al. Identification of a novel, small molecule inhibitor of KCNQ2 channels. Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US) 2010–2011 Oct 28.

  21. Li Q, Rottlander M, Xu M, Christoffersen CT, Frederiksen K, Wang MW, et al. Identification of novel KCNQ4 openers by a high-throughput fluorescence-based thallium flux assay. Anal Biochem 2011; 418: 66–72.

    CAS  Article  PubMed  Google Scholar 

  22. Ion channel screening – automated patch clamp on the rise. Drug Discov Today Technol 2008; 5 e1–e34.

  23. Lepple-Wienhues A, Lassen D, Hummer A, Czubayko U, Knirsch M, Golubovic A, et al. The Chiptip: a novel tool for automated patch clamp. Comb Chem High Throughput Screen 2009; 12: 73–7.

    CAS  Article  PubMed  Google Scholar 

  24. Shieh CC . Automated high-throughput patch-clamp techniques. Drug Discov Today 2004; 9 551–2.

    Article  PubMed  Google Scholar 

  25. Yu H, Wu M, Hopkins C, Engers J, Townsend S, Lindsley C, et al. A small molecule activator of KCNQ2 and KCNQ4 channels. Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US) 2010–2011 Mar 29.

  26. Mattmann ME, Yu H, Lin Z, Xu K, Huang X, Long S, et al. Identification of (R)-N-(4-(4-methoxyphenyl)thiazol-2-yl)-1-tosylpiperidine-2-carboxamide, ML277, as a novel, potent and selective K(v)7.1 (KCNQ1) potassium channel activator. Bioorg Med Chem Lett 2012; 22 5936–41.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  27. Py C, Martina M, Diaz-Quijada GA, Luk CC, Martinez D, Denhoff MW, et al. From understanding cellular function to novel drug discovery: the role of planar patch-clamp array chip technology. Front Pharmacol 2011; 2: 51.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  28. Carmelle V . Conventional Patch Clamp Techniques and High-Throughput Patch Clamp Recordings on a Chip for Measuring Ion Channel Activity, in Textbook of Pulmonary Vascular Disease 2010; Springer. USA. P 495–510.

  29. Schroeder K, Neagle B, Trezise DJ, Worley J . Ionworks HT: a new high-throughput electrophysiology measurement platform. J Biomol Screen 2003; 8 50–64.

    CAS  Article  PubMed  Google Scholar 

Download references

Acknowledgements

We gratefully acknowledge the financial support from the National Natural Science Foundation of China Grant for Excellent Key Laboratory (81123004), the National Natural Science Foundation of China (61327014, 61175103 and 91413122), Shanghai Municipal Science and Technology Commission (13JC1406700) and the External Cooperation Program of BIC, Chinese Academy of Sciences (1536631KYSB20130003).

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Fa-jun Nan or Zhao-bing Gao.

Additional information

Supplementary Table S1 is available at Acta Pharmacologica Sinica's website.

Supplementary information

Supplementary Information, Table S1

Potentiation activity of validated hits on KCNQ2 currents. (DOCX 252 kb)

PowerPoint slides

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Yue, Jf., Qiao, Gh., Liu, N. et al. Novel KCNQ2 channel activators discovered using fluorescence-based and automated patch-clamp-based high-throughput screening techniques. Acta Pharmacol Sin 37, 105–110 (2016). https://doi.org/10.1038/aps.2015.142

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/aps.2015.142

Keywords

  • KCNQ2
  • channel activator
  • high-throughput screening
  • thallium flux assay
  • automated patch-clamp
  • ZG1732
  • ZG2083

Further reading

Search

Quick links