Abstract
Aim:
To evaluate the antiapoptotic effect of the A20 gene in primary hippocampal neurons both in vivo and in vitro.
Methods:
Primary hippocampal neurons in embryonic day 18 (E18) rats were transfected with the A20 gene by using the new Nucleofector electroporation transfection method. We then examined, whether A20 -neurons possessed anti-apoptotic abilities after TNF-α stimulation in vitro. A20-neurons and pcDNA3 -neurons were transplanted into the penumbra of the brains of rats that had been subjected to 90-min of ischemia induced by left middle cerebral artery occlusion (MCAO).
Results:
A20-neurons resisted TNF-α induced apoptosis in vitro. The apoptosis rate of neurons overexpressing A20 (28.46%±3.87%) was lower than that in neurons transfected with pcDNA3 (53.06%±5.36%). More A20-neurons survived in the penumbra both 3-d and 7-d after transplantation than did sham pcDNA3 neurons.
Conclusion:
The novel function of A20 may make it a potential targets for the gene therapy for neurological diseases.
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Project supported by Major State Basic Research Development Program of China (No G1999053905).
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Miao, Hs., Yu, Ly., Hui, Gz. et al. Antiapoptotic effect both in vivo and in vitro of A20 gene when transfected into rat hippocampal neurons. Acta Pharmacol Sin 26, 33–38 (2005). https://doi.org/10.1111/j.1745-7254.2005.00002.x
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DOI: https://doi.org/10.1111/j.1745-7254.2005.00002.x