Abstract
Aim:
To investigate the inhibitory effect of a new compound of GLB on tumor metastasis in vivo and analyze its actions on tumor cell adhesion to clarify its mechanism.
Methods:
The effect of GLB on tumor metastasis was analyzed by Lewis lung carcinoma model. The pathological morphology of lung alveolar was evaluated by hematoxylin-eosin staining. The effect of GLB on the proliferation of human prostate cancer cell (PC-3M, with a high metastatic characteristic) was studied using the MTT method, and its actions on PC-3M cell adhesion to human umbilical vein endothelial cells (HUVEC) and laminin were analyzed in vitro.
Results:
GLB (100 mg·kg−1·d−1 for 28 d, ig) reduced the number of lung colonies of Lewis lung carcinoma metastasis significantly (P < 0.05). Simultaneously, GLB could mitigate the damage of lung alveolar caused by metastasic tumor deposits. In vitro, GLB inhibited dramatically the adhesion of PC-3M cells to HUVEC (P < 0.01) and laminin (P < 0.05), without cytotoxic or anti-proliferative action on PC-3M cells.
Conclusion:
GLB has anti-tumor metastatic activity, which partly depends on its inhibition of tumor adhesion.
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Project supported by the National Natural Science Foundation of China (No 39770286, 30171090) and 973 Program of the Ministry of Science and Technology (No 2004CB518902).
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Pan, Y., Song, Ql., Lin, Yh. et al. GLB prevents tumor metastasis of Lewis lung carcinoma by inhibiting tumor adhesion actions. Acta Pharmacol Sin 26, 881–886 (2005). https://doi.org/10.1111/j.1745-7254.2005.00125.x
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DOI: https://doi.org/10.1111/j.1745-7254.2005.00125.x
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