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Analytical biochemistry

Weighing up protein folding

Labelling molecules by fast oxidation allows mass spectrometry to study protein folding at submillisecond time resolution. The method also brings a wealth of structural information about protein folding within reach.

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Figure 1: Improving the time resolution of mass spectrometry in protein-folding studies.


  1. Kubelka, J., Hofrichter, J. & Eaton, W. A. Curr. Opin. Struct. Biol. 14, 76–88 (2004).

    CAS  Article  Google Scholar 

  2. Chen, J., Rempel, D. L. & Gross, M. L. J. Am. Chem. Soc. 132, 15502–15504 (2010).

    CAS  Article  Google Scholar 

  3. Shaw, D. E. et al. Science 330, 341–346 (2010).

    ADS  CAS  Article  Google Scholar 

  4. Gruebele, M. C.R. Biol. 328, 701–712 (2005).

    CAS  Article  Google Scholar 

  5. Nölting, B., Golbik, R. & Fersht, A. R. Proc. Natl Acad. Sci. USA 92, 10668–10672 (1995).

    ADS  Article  Google Scholar 

  6. Ballew, R. M., Sabelko, J. & Gruebele, M. Proc. Natl Acad. Sci. USA 93, 5759–5764 (1996).

    ADS  CAS  Article  Google Scholar 

  7. Gau, B. C., Sharp, J. S., Rempel, D. L. & Gross, M. L. Anal. Chem. 81, 6563–6571 (2009).

    CAS  Article  Google Scholar 

  8. de Laureto, P. P., De Filippis, V., Di Bello, M., Zambonin, M. & Fontana, A. Biochemistry 34, 12596–12604 (1995).

    Article  Google Scholar 

  9. Tsui, V. et al. Protein Sci. 8, 45–49 (1999).

    CAS  Article  Google Scholar 

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Gruebele, M. Weighing up protein folding. Nature 468, 640–641 (2010).

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