Abstract
The CD5+ mouse pre-B cell line SPGM-1 is able to undergo lineage conversion towards a macrophage like cell type. Although one of the κ-light chain alleles is rearranged in V-Jκ4 configuration, no protein is expressed nor could the expression be induced. Infection with and expression of the human bcl-2 gene protected SPGM-1 cells from apoptosis, allowing the rearrangement of their second κ-allele in V-Jκ5 configuration. mRNA transcripts of the V-Jκ regions were detected only in SPGM-1×bcl-2 cells, but not in SPGM-1, while κ-light chain protein was not found in any of the cell lines. The myeloid differentiation potential of SPGM-1 cells was not affected by overexpression of the human bcl-2 gene. Upon appropriate stimulation, SPGM-1xbcl-2 cells became enlarged, adhered to the plastic surfaces and lost their immunoglobulin μ heavy chain expression.
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Neumann, D., Strasser, A., Wesche, H. et al. Overexpression of the bcl-2 oncogene in the mouse pre-B cell line SPGM-1 protects from apoptosis, but does not affect blocked B-lineage differentiation and lineage switch towards macrophage like cells. Cell Death Differ 4, 580–589 (1997). https://doi.org/10.1038/sj.cdd.4400279
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DOI: https://doi.org/10.1038/sj.cdd.4400279