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Immunology

Hepatitis A virus link to atopic disease

Abstract

Atopic diseases, including asthma, allergic rhinitis and atopic dermatitis, are caused by both environmental and genetic factors. Here we show that infection by hepatitis A virus (HAV) may protect individuals from atopy if they carry a particular variant of the gene that encodes TIM-1 (also known as HAVcr-1) — the cell-surface receptor used by HAV to infect human cells1. Exposure to HAV is associated with poor hygiene, large family size and attendance at day-care centres, all factors that are also inversely associated with atopy2,3,4,5,6. Our discovery indicates that interaction between HAV and TIM-1 genotype may contribute to the aetiology of atopic diseases, and provides a mechanism to account for the hygiene hypothesis.

Main

Using a congenic positional cloning strategy, we identified TIM-1 as a candidate gene for atopy and asthma in a region of mouse chromosome 11, which is homologous to a segment of human chromosome 5q31–33 that has been linked to atopy7,8. TIM-1 is expressed by activated CD4+ T cells during the development of helper-T-cell (Th2) responses and regulates cytokine production7. We therefore investigated whether the interaction between HAV and TIM-1 on lymphocytes can modify T cells in a way that protects against atopy, and whether polymorphisms in TIM-1 can alter susceptibility to atopy7.

By sequencing complementary DNA from human lymphocytes, we identified a six-amino-acid insertion (ins) at residue 157, termed 157insMTTTVP (one-letter amino-acid notation), as well as two single-amino-acid changes, 195delT (where 'del' signifies a deletion) and A206T. The insertion 157insMTTTVP is located at the centre of an extracellular mucin-like region that is required for efficient HAV uncoating9 and, because 157insMTTTVP lengthens this critical region by 12–14%, this variation may affect the efficiency of viral entry (Fig. 1).

Figure 1: Possible mechanisms of interaction between the cell-surface TIM-1 receptor and hepatitis A virus (HAV), and the effect of HAV on cytokine production.
figure1

a, A variant of TIM-1 ('hook') that carries a 6-amino-acid insert (red), 157insMTTTVP, may increase binding of HAV (large pentagons) to the receptor, thereby enhancing HAV viral uncoating (small pentagons) and infection of TIM-1-expressing T cells. This could lead to deletion of certain lymphocyte subsets, such as Th2 cells, or reduce Th2-cell differentiation, causing a reduction in atopy and asthma. b, Alternatively, HAV may bind less efficiently to the form of TIM-1 without the insertion, resulting in less HAV infection and hence more Th2-cell development and more atopy. The mechanism that underpins this interaction between TIM-1, its 157insMTTTVP region and HAV could relate to viral uncoating9, the extent and duration of HAV viraemia10, or to a direct effect on Th1/Th2-subset differentiation.

To determine the effect of the insertion 157insMTTTVP on the occurrence of atopy, we carried out a cross-sectional study of 375 individuals who were evaluated by history and tested serologically for atopy and prior HAV infection. To correct for potentially confounding effects of population admixture, we used stratified Mantel–Haenszel χ2 tests to quantify the association between atopy and 157insMTTTVP in the total sample. We found that HAV seropositivity protects against atopy, but only in individuals with the 157insMTTTVP variant of TIM-1 (P = 0.0005; Table 1).

Table 1 TIM-1 insert and protection against atopy

The protective effects of HAV therefore depend upon a common TIM-1 allele that is carried by 63% of Caucasians, 46% of Asians and 64% of African Americans in this population (see supplementary information). As allelic variation in TIM-1 does not affect HAV-infection rates in our population (χ2 = 1.567, P = 0.211), we conclude that the interaction of HAV with TIM-1 genotype seen here is not due to variation in the rate of seroconversion following HAV exposure.

Before 1970, the seroprevalence of antibodies against HAV approached 100% in Western countries4, and infection with HAV may have protected many individuals against atopy3. However, modernization has led to a reduction in average family size and significant improvements in public health, causing anti-HAV seroprevalence to fall to 25–30%, while the prevalence of atopic disease has doubled4. Our finding that TIM-1 is associated with atopy in HAV-seropositive individuals indicates that exposure to a specific pathogen may influence the expression of atopy — so a declining prevalence of HAV infection could contribute to an increase in atopy by association with TIM-1. It will be necessary to determine whether HAV exposure must occur during childhood to have a protective effect, whether HAV can mitigate the severity of existing atopic disease, and whether HAV vaccination can reproduce the effects of natural HAV infection.

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Correspondence to Dale T. Umetsu.

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The authors declare no competing financial interests.

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McIntire, J., Umetsu, S., Macaubas, C. et al. Hepatitis A virus link to atopic disease. Nature 425, 576 (2003). https://doi.org/10.1038/425576a

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