Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Computational biology

Biosensor design

A series of bacterial receptor proteins have been 'redesigned' by computer so that they bind molecules that are quite different from their natural ligands. The approach might be useful for designing catalytic proteins.

Your institute does not have access to this article

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

Figure 1: Computational design of binding proteins.

References

  1. Looger, L. L., Dwyer, M. A., Smith, J. J. & Hellinga, H. W. Nature 423, 185–190 (2003).

    ADS  CAS  Article  Google Scholar 

  2. Hoess, R. Chem. Rev. 101, 3205–3218 (2001).

    CAS  Article  Google Scholar 

  3. Dower, W. J. & Mattheakis, L. C. Curr. Opin. Biotechnol. 6, 390–398 (2002).

    CAS  Article  Google Scholar 

  4. Tam, R. & Saier, M. H. Jr Microbiol. Rev. 57, 320–346 (1993).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Desjarlais, J. R. & Clarke, N. D. Curr. Opin. Struct. Biol. 8, 471–475 (1998).

    CAS  Article  Google Scholar 

  6. Lasters, I., De Maeyer, M. & Desmet, J. Protein Eng. 8, 815–822 (1995).

    CAS  Article  Google Scholar 

  7. Dahiyat, B. I. & Mayo, S. L. Science 278, 82–87 (1997).

    CAS  Article  Google Scholar 

  8. Ghirlanda, G., Lear, J. D., Lombardi, A. & DeGrado, W. F. J. Mol. Biol. 281, 379–391 (1998).

    CAS  Article  Google Scholar 

  9. Reina, J. et al. Nature Struct. Biol. 9, 621–627 (2002).

    CAS  PubMed  Google Scholar 

  10. Wentworth, P. Jr & Janda, K. D. Cell Biochem. Biophys. 35, 63–87 (2001).

    CAS  Article  Google Scholar 

  11. Bolon, D. N. & Mayo, S. Proc. Natl Acad. Sci. USA 98, 14272–14279 (2001).

    ADS  Article  Google Scholar 

  12. Baltzer, L. & Nilsson, J. Curr. Opin. Biotechnol. 12, 355–360 (2001).

    CAS  Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to William F. DeGrado.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

DeGrado, W. Biosensor design. Nature 423, 132–133 (2003). https://doi.org/10.1038/423132a

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/423132a

Further reading

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing