Chromatin–IgG complexes activate B cells by dual engagement of IgM and Toll-like receptors

Abstract

Autoreactive B cells are present in the lymphoid tissues of healthy individuals, but typically remain quiescent. When this homeostasis is perturbed, the formation of self-reactive antibodies can have serious pathological consequences. B cells expressing an antigen receptor specific for self-immunoglobulin-γ (IgG) make a class of autoantibodies known as rheumatoid factor (RF). Here we show that effective activation of RF+ B cells is mediated by IgG2a–chromatin immune complexes and requires the synergistic engagement of the antigen receptor and a member of the MyD88-dependent Toll-like receptor (TLR) family. Inhibitor studies implicate TLR9. These data establish a critical link between the innate and adaptive immune systems in the development of systemic autoimmune disease and explain the preponderance of autoantibodies reactive with nucleic acid–protein particles. The unique features of this dual-engagement pathway should facilitate the development of therapies that specifically target autoreactive B cells.

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Figure 1: Anti-nucleosome antibody stimulation of RF+ B cells is DNase sensitive.
Figure 2: Anti-TNP–TNP-BSA immune complexes fail to efficiently stimulate proliferation of RF+ B cells.
Figure 3: Stimulation of RF+ B cells by autoantibody–autoantigen immune complex is not dependent on the complement receptor.
Figure 4: Stimulation of RF+ B cells by autoantibody–autoantigen immune complex is dependent on MyD88.
Figure 5: Stimulation of RF+ B cells by autoantibody–autoantigen immune complexes can be blocked by inhibitors of the TLR9 signalling pathway.

References

  1. 1

    Tan, E. Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology. Adv. Immunol. 44, 93–151 (1989)

    CAS  Article  Google Scholar 

  2. 2

    Theofilopoulos, A. N. et al. Association of lpr gene with graft-vs-host disease-like syndrome. J. Exp. Med. 162, 1–18 (1985)

    CAS  Article  Google Scholar 

  3. 3

    Wolfowicz, C. B., Sakorafas, P., Rothstein, T. L. & Marshak-Rothstein, A. Oligoclonality of rheumatoid factors arising spontaneously in lpr/lpr mice. Clin. Immunol. Immunopathol. 46, 382–395 (1988)

    CAS  Article  Google Scholar 

  4. 4

    Shlomchik, M. J., Zharhary, D., Camper, S., Saunders, T. & Weigert, M. A rheumatoid factor transgenic mouse model for autoantibody regulation. Int. Immunol. 5, 1329–1341 (1993)

    CAS  Article  Google Scholar 

  5. 5

    Jacobson, B. A. et al. An isotype switched and somatically mutated rheumatoid factor clone isolated from a MRL-lpr/lpr mouse exhibits limited intraclonal affinity maturation. J. Immunol. 152, 4489–4499 (1994)

    CAS  PubMed  Google Scholar 

  6. 6

    Hannum, L. G., Ni, D., Haberman, A. M., Weigert, M. G. & Shlomchik, M. J. A disease-related rheumatoid factor autoantibody is not tolerized in a normal mouse: implications for the origins of autoantibodies in autoimmune disease. J. Exp. Med. 184, 1269–1278 (1996)

    CAS  Article  Google Scholar 

  7. 7

    Wang, H. & Shlomchik, M. J. Autoantigen-specific B cell activation in Fas-deficient rheumatoid factor immunoglobulin transgenic mice. J. Exp. Med. 190, 639–649 (1999)

    CAS  Article  Google Scholar 

  8. 8

    Rifkin, I. R. et al. Immune complexes present in the sera of autoimmune mice activate rheumatoid factor B cells. J. Immunol. 165, 1626–1633 (2000)

    CAS  Article  Google Scholar 

  9. 9

    Emlen, W., Holers, V. M., Arend, W. P. & Kotzin, B. Regulation of nuclear antigen expression on the cell surface of human monocytes. J. Immunol. 148, 3042–3048 (1992)

    CAS  PubMed  Google Scholar 

  10. 10

    Monestier, M. & Novick, K. E. Specificities and genetic characteristics of nucleosome-reactive antibodies from autoimmune mice. Mol. Immunol. 33, 89–99 (1996)

    CAS  Article  Google Scholar 

  11. 11

    Carter, R. H., Spycher, M. O., Ng, Y. C., Hoffman, R. & Fearon, D. T. Synergistic interaction between complement receptor type 2 and membrane IgM on B lymphocytes. J. Immunol. 141, 457–463 (1988)

    CAS  Google Scholar 

  12. 12

    Ahearn, J. M. et al. Disruption of the Cr2 locus results in a reduction in B-1a cells and an impaired B cell response to T-dependent antigen. Immunity 4, 251–262 (1996)

    CAS  Article  Google Scholar 

  13. 13

    Lemaitre, B., Nicholas, E., Michaut, L., Reichhart, J. M. & Hoffmann, J. A. The dorsoventral regulatory gene cassette spatzle/Toll/cactus controls the potent antifungal response in Drosophila adults. Cell 86, 973–983 (1996)

    CAS  Article  Google Scholar 

  14. 14

    Medzhitov, R., Preston-Hurlburt, P. & Janeway, C. A. A human homologue of the Drosophila Toll protein signals activation of adaptive autoimmunity. Nature 388, 323–324 (1997)

    Article  Google Scholar 

  15. 15

    Akira, S., Takeda, K. & Kaisho, T. Toll-like receptors: critical proteins linking innate and acquired immunity. Nature Immunol. 2, 675–680 (2001)

    CAS  Article  Google Scholar 

  16. 16

    Li, M. et al. An essential role of the NF-κB/Toll-like receptor pathway in induction of inflammatory and tissue-repair gene expression by necrotic cells. J. Immunol. 166, 7128–7135 (2001)

    CAS  Article  Google Scholar 

  17. 17

    Horng, T., Barton, G. M. & Medzhitov, R. TIRAP: an adapter molecule in the Toll signalling pathway. Nature Immunol. 2, 835–841 (2001)

    CAS  Article  Google Scholar 

  18. 18

    Adachi, O. et al. Targeted disruption of the Myd88 gene results in loss of IL-1 and IL-18-mediated function. Immunity 9, 143–150 (1998)

    CAS  Article  Google Scholar 

  19. 19

    Hacker, H. et al. Immune cell activation by bacterial CpG-DNA through myeloid differentiation marker 88 and tumour necrosis factor receptor-associated factor (TRAF)6. J. Exp. Med. 192, 595–600 (2000)

    CAS  Article  Google Scholar 

  20. 20

    Singal, R. & Ginder, G. D. DNA methylation. Blood 93, 4059–4070 (1999)

    CAS  Google Scholar 

  21. 21

    Hemmi, H. et al. A Toll-like receptor recognizes bacterial DNA. Nature 408, 740–745 (2000)

    ADS  CAS  Article  Google Scholar 

  22. 22

    Yi, A.-K. et al. CpG motifs in bacterial DNA activate leukocytes through the pH-dependent generation of reactive oxygen species. J. Immunol. 160, 4755–4761 (1998)

    CAS  PubMed  PubMed Central  Google Scholar 

  23. 23

    Hacker, H. et al. CpG-DNA-specific activation of antigen-presenting cells requires stress kinase activity and is preceded by non-specific endocytosis and endosomal maturation. EMBO J. 17, 6230–6240 (1998)

    CAS  Article  Google Scholar 

  24. 24

    Benaroch, P. et al. How MHC class II molecules reach the endocytic pathway. EMBO J. 14, 37–49 (1995)

    CAS  Article  Google Scholar 

  25. 25

    Massari, P. et al. Immune stimulation by neisserial porins is TLR2 and MyD88-dependent. J. Immunol. 168, 1533–1537 (2002)

    CAS  Article  Google Scholar 

  26. 26

    The Canadian Hydroxychloroquine Study Group. A randomized study of the effect of withdrawing hydroxychloroquine sulfate in systemic lupus erythematosus. N. Engl. J. Med. 324, 150–154 (1991)

    Article  Google Scholar 

  27. 27

    Furst, d. E. et al. Dose-loading with hydroxychloroquine improves the rate of response in early, active rheumatoid arthritis. Arthritis Rheum. 42, 357–365 (1999)

    CAS  Article  Google Scholar 

  28. 28

    Lenart, P., Stunz, L., Yi, A.-K., Krieg, A. M. & Ashman, R. F. CpG stimulation of primary mouse B cells is blocked by inhibitory oligodeoxyribonucleotides at a site proximal to NF-κB activation. Antisense Nucleic Acid Drug Dev. 4, 247–256 (2001)

    Article  Google Scholar 

  29. 29

    Shlomchik, M. J., Marshak-Rothstein, A., Wolfowicz, C. B., Rothstein, T. L. & Weigert, M. G. The role of clonal selection and somatic mutation in autoimmunity. Nature 328, 805–811 (1987)

    ADS  CAS  Article  Google Scholar 

  30. 30

    Zeng, D., Lee, M.-K., Tung, J., Brendolan, A. & Strober, S. Cutting edge: A role for CD1 in the pathogenesis of Lupus in NZB/NZW mice. J. Immunol. 164, 5000–5004 (2000)

    CAS  Article  Google Scholar 

  31. 31

    William, J., Christensen, C. & Shlomchick, M. J. Evolution of an autoantibody response is linked to somatic hypermutation outside of germinal centers. (submitted).

  32. 32

    Botto, M. et al. Homozygous C1q deficiency causes glomerulonephritis associated with multiple apoptotic bodies. Nature Genet. 19, 56–59 (1998)

    CAS  Article  Google Scholar 

  33. 33

    Bickerstaff, M. C. M. et al. Serum amyloid P component controls chromatin degradation and prevents antinuclear autoimmunity. Nature Med. 5, 694–697 (1999)

    CAS  Article  Google Scholar 

  34. 34

    Napirei, M. et al. Features of systemic lupus erythematosis in DNase1-deficient mice. Nature Genet. 25, 177–180 (2000)

    CAS  Article  Google Scholar 

  35. 35

    Scott, R. S. et al. Phagocytosis and clearance of apoptotic cells is mediated by MER. Nature 411, 207–211 (2001)

    ADS  CAS  Article  Google Scholar 

  36. 36

    Bell, D. A., Morrison, B. & VandenBygaart, P. Immunogenic DNA-related factors. Nucleosomes spontaneously released from normal murine lymphoid cells stimulate proliferation and immunoglobulin synthesis of normal mouse lymphocytes. J. Clin. Invest. 85, 1487–1496 (1990)

    CAS  Article  Google Scholar 

  37. 37

    Bell, D. A. & Morrison, B. The spontaneous apoptotic death of normal human lymphocytes in vitro: the release of, and immunoproliferative response to, nucleosomes in vitro. Clin. Immunol. Immunopathol. 60, 1326 (1991)

    Article  Google Scholar 

  38. 38

    Moller, G., Andersson, J. & Sjoberg, O. Lipopolysaccharides can convert heterologous red cells into thymus-independent antigens. Cell. Immunol. 4, 416–424 (1972)

    CAS  Article  Google Scholar 

  39. 39

    Kawai, T., Adachi, O., Ogawa, T., Takeda, K. & Akira, S. Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity 11, 115–122 (1999)

    CAS  Article  Google Scholar 

Download references

Acknowledgements

We thank M. Carroll, S. Akira and D. Golenbock for providing the Cr2-deficient and MyD88-deficient mice; M. Boulé, C. Chi, C. Lau and G. Yospin for technical assistance; L. Wetzler and D. Golenbock for providing TLR ligands; H. Ploegh for providing concanamycin B; M. Fenton, L. Wetzler, R. Corley, R. Medzhitov, T. Rothstein and D. Stollar for reviewing the manuscript and/or discussions. This work was supported by grants from the National Institutes of Health, the Arthritis Foundation, and the National Kidney Foundation.

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A provisional patent on the use of TLR inhibitors in the treatment of autoimmune disease.

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Leadbetter, E., Rifkin, I., Hohlbaum, A. et al. Chromatin–IgG complexes activate B cells by dual engagement of IgM and Toll-like receptors. Nature 416, 603–607 (2002). https://doi.org/10.1038/416603a

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