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Inter-receptor communication through arrays of bacterial chemoreceptors

Nature volume 415, pages 8184 (03 January 2002) | Download Citation

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Abstract

The sensing mechanisms of chemotactic bacteria allow them to respond sensitively to stimuli. Escherichia coli, for example, respond to changes in chemoattractant concentration of less than 10% over a range spanning six orders of magnitude1,2. Sensitivity over this range depends on a nonlinear relationship between ligand concentration and output response3. At low ligand concentrations, substantial amplification of the chemotactic signal is required; however, the mechanism responsible for this amplification remains unclear. Here we demonstrate that inter-receptor communication within a lattice4,5 acts to amplify and integrate sensory information. Synthetic multivalent ligands that interact through the low-abundance, galactose-sensing receptor Trg stabilize large clusters of chemoreceptors and markedly enhance signal output from these enforced clusters. On treatment with multivalent ligands, the response to the attractant serine is amplified by at least 100-fold. This amplification requires a full complement of chemoreceptors; deletion of the aspartate (Tar) or dipeptide (Tap) receptors diminishes the amplification of the serine response. These results demonstrate that the entire array is involved in sensing. This mode of information exchange has general implications for the processing of signals by cellular receptors.

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Acknowledgements

We thank J. Adler for supplying E. coli strains AW405, AW550, AW518 and AW701, and for advice. We thank J. S. Parkinson for E. coli strains RP1078, RP5854 and RP2361, and for anti-MCP antibodies. This work was supported by the National Institutes of Health (NIH). J.E.G. acknowledges the NIH Biotechnology Training Grant for support.

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  1. Departments of Chemistry and Biochemistry, University of Wisconsin–Madison, Madison, Wisconsin 53706, USA

    • Jason E. Gestwicki
    •  & Laura L. Kiessling

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The authors declare no competing financial interests.

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Correspondence to Laura L. Kiessling.

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https://doi.org/10.1038/415081a

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