Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

An RNA-dependent ATPase associated with U2/U6 snRNAs in pre-mRNA splicing

Nature volume 381, pages 709–713 (1996)Cite this article

Abstract

THE hydrolysis of ATP by a group of RNA-dependent ATPases (DEAD/H proteins1) is required for spliceosome assembly, but not for the subsequent transesterification reactions2. Little is known about the function of these ATPases in relation to the RNA conformational changes that occur in formation of active structures, in which U2/U6 small nuclear RNA (snRNA) interactions3,4 are essential for splicing to take place. Using a synthetic lethal genetic screen, we have isolated four yeast splicing factors involved in U2/U6 snRNA interactions (D.X. et al., manuscript in preparation). The RNA-dependent ATPase activity associated with one such factor, the Slt22 protein, is stimulated preferentially by annealed U2/U6 snRNAs. Both mutant slt22-1 and U2 snRNA cause a reduction in stimulation. The slt22-1 mutation blocks splicing at or before the first step, resulting in the accumulation of an unusual complex which lacks U5 snRNA. Our results indicate that the U2/U6 snRNA interactions facilitated by Slt22 are also involved in the interaction of U5 snRNA with the spliceosome.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Schmid, S. R. & Linder, P. Molec. Microbiol. 6, 283–292 (1992).

    Article  CAS  Google Scholar 

  2. Moore, M. J. et al. in The RNA World (eds Gesteland, R. F. & Atkins, J. F.) 303–357 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1993).

    Google Scholar 

  3. Madhani, H. D. & Guthrie, C. Cell 71, 803–817 (1992).

    Article  CAS  Google Scholar 

  4. Sun, J.-S. & Manley, J. L. Genes Dev. 9, 843–854 (1995).

    Article  CAS  Google Scholar 

  5. Madhani, H. D. & Guthrie, C. A. Rev. Genet. 28, 1–26 (1994).

    Article  CAS  Google Scholar 

  6. Pause, A., Methot, N. & Sonenberg, N. Molec. cell. Biol. 13, 6789–6798 (1993).

    Article  CAS  Google Scholar 

  7. Cheng, S. C. & Abelson, J. Genes Dev. 1, 1014–1027 (1987).

    Article  CAS  Google Scholar 

  8. Burgess, S. M. & Guthrie, C. Cell 73, 1377–1391 (1993).

    Article  CAS  Google Scholar 

  9. Burgess, S. M. & Guthrie, C. Trends biochem. Sci. 18, 381–384 (1993).

    Article  CAS  Google Scholar 

  10. Lauber, J. et al. EMBO J. (in the press).

  11. Frank, D., Patterson, B. & Guthrie, C. Molec. cell. Biol. 12, 5197–5205 (1992).

    Article  CAS  Google Scholar 

  12. Jones, M. H., Frank, D. N. & Guthrie, C. Proc. natn. Acad. Sci. U.S.A. 92, 9687–9691 (1995).

    Article  ADS  CAS  Google Scholar 

  13. Brown, J. D. & Beggs, J. D. EMBO J. 11, 3721–3729 (1992).

    Article  CAS  Google Scholar 

  14. Field, D. & Friesen, J. D. Genes Dev. 10, 489–501 (1996).

    Article  CAS  Google Scholar 

  15. Lin, R.-J. et al. J. biol. Chem. 260, 14780–14792 (1985).

    CAS  PubMed  Google Scholar 

  16. Rose, M. D. & Broach, J. R. Meth. Enzym. 194, 195–230 (1991).

    Article  CAS  Google Scholar 

  17. Kolodzie, P. A. & Young, R. Meth. Enzym. 194, 508–519 (1991).

    Article  Google Scholar 

  18. McPheeters, D. S. & Abelson, J. Cell 71, 819–831 (1992).

    Article  CAS  Google Scholar 

  19. Fuller-Pace, F. V. et al. EMBO J. 12, 3619–3626 (1993).

    Article  CAS  Google Scholar 

  20. Tarn, W.-Y. et al. Proc. natn. Acad. Sci. U.S.A. 90, 10821–10825 (1993).

    Article  ADS  CAS  Google Scholar 

  21. Fabrizio, P. & Abelson, J. Science 250, 404–409 (1990).

    Article  ADS  CAS  Google Scholar 

  22. Madhani, H. D. et al. Genes Dev. 4, 2264–2277 (1990).

    Article  CAS  Google Scholar 

  23. McPhoeters, D. S. & Abelson, J. et al. Cell 71, 819–831 (1992).

    Article  Google Scholar 

  24. Frank, D. N., Roiha, H. & Guthrie, C. Molec. cell. Biol. 14, 2180–2190 (1994).

    Article  CAS  Google Scholar 

  25. Newman, A. J., Teigelkamp, S. & Beggs, J. D. RNA 1, 968–980 (1995).

    CAS  PubMed  PubMed Central  Google Scholar 

  26. Madhani, H. D. & Guthrie, C. Genes Dev. 8, 1071–1086 (1994).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Deming Xu, Shahrzad Nouraini, Deborah Field, Shou-Jiang Tang & James D. Friesen

    Present address: Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario, M5G 1L6, Canada

Authors and Affiliations

  1. Department of Genetics, Research Institute, Hospital for Sick Children,

    Deming Xu, Shahrzad Nouraini, Deborah Field, Shou-Jiang Tang & James D. Friesen

  2. Department of Molecular and Medical Genetics, University of Toronto, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada

    Deming Xu, Shahrzad Nouraini, Deborah Field, Shou-Jiang Tang & James D. Friesen

Authors
  1. Deming Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shahrzad Nouraini
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Deborah Field
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shou-Jiang Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. James D. Friesen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, D., Nouraini, S., Field, D. et al. An RNA-dependent ATPase associated with U2/U6 snRNAs in pre-mRNA splicing. Nature 381, 709–713 (1996). https://doi.org/10.1038/381709a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/381709a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing