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An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast

Nature volume 379, pages 180–182 (1996)Cite this article

Abstract

ORIGINS of DNA replication in Saccharomyces cerevisiae are bound by two protein complexes during the cell cycle1,2. Post-replicative complexes closely resemble those generated in vitro by purified origin recognition complex (ORC)1,3–5, which is essential for DNA replication in vivo6–11. Pre-replicative complexes (pre-RCs) are characterized by an extended region of nuelease protection overlapping the ORC footprint1. We show here that the Cdc6 protein (Cdc6p), which is necessary for origin firing in vivo12–16, is essential for the establishment and maintenance of pre-RCs, suggesting that it is a component of these complexes. Without Cdc6p, G1 origins closely resemble post-replicative origins, providing evidence that ORC is also a component of pre-RCs. These results suggest that pre-RCs play an essential role in initiating DNA replication and support a two-step mechanism for the assembly of functional initiation complexes.

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Author notes

  1. John F. X. Diffley: To whom correspondence should be addressed

Authors and Affiliations

  1. Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, UK

    Julie H. Cocker, Corrado Santocanale & John F. X. Diffley

  2. Research Institute of Molecular Pathology, Dr. Bohr Gasse 7, 1030, Vienna, Austria

    Simonetta Piatti & Kim Nasmyth

Authors
  1. Julie H. Cocker
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  2. Simonetta Piatti
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  3. Corrado Santocanale
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  4. Kim Nasmyth
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  5. John F. X. Diffley
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Cocker, J., Piatti, S., Santocanale, C. et al. An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast. Nature 379, 180–182 (1996). https://doi.org/10.1038/379180a0

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