Fluorescence polarization (FP) equilibrium binding assays differ from other types of binding studies in one important regard: they require no steps to separate free from bound tracer and are therefore fast, simple and accurate.
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Checovich, W., Bolger, R. & Burke, T. Fluorescence polarization — a new tool for cell and molecular biology. Nature 375, 254–256 (1995). https://doi.org/10.1038/375254a0
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DOI: https://doi.org/10.1038/375254a0
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