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Crystal structure of the extracellular region of human tissue factor

Nature volume 370, pages 662–666 (1994)Cite this article

An Erratum to this article was published on 20 October 1994

Abstract

TISSUE factor is a cell-surface glycoprotein receptor which initiates the blood coagulation cascade after vessel injury by interacting with blood clotting factor VH/VIIa and which is implicated in various pathological processes1. When bound to tissue factor, factor VII is readily converted to the active protease factor Vila by trace amounts of factors Xa, IXa or Vila. Human tissue factor consists of 263 residues, the first 219 of which comprise the extracellular region2. We have determined the ocrystal structure of the extracellular region at a resolution of 2.2 Å. Tissue factor consists of two immunoglobulin-like domains associated through an extensive, novel, interdomain interface region. The binding site for factor VII lies at the interface region and involves residues from domain 1 and an extended loop (binding ‘finger’) of domain 2. This is the first reported structure of a representative of the class 2 cytokine receptor family, which also includes interferon-α, interferon-γ (refs 2, 3) and interleukin-10 (ref. 4) receptors.

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Author information

Author notes

  1. D. M. A. Martin, D. P. O'Brien & E. G. D. Tuddenham

    Present address: Haemostasis Research Group, MRC Clinical Sciences Centre, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London, W12 ONN, UK

  2. A. Miller

    Present address: Principal's Office, University of Sterling, Sterling, FK9 4LA, UK

Authors and Affiliations

  1. Laboratory of Molecular Biophysics and Oxford Centre for Molecular Sciences, Rex Richards Building, South Parks Road, Oxford, 0X1 3QU, UK

    K. Harlos, E. Y. Jones & D. I. Stuart

  2. Haemostasis Research Group, MRC Clinical Research Centre, Watford Road, Harrow, Middlesex, HA1 3UJ, UK

    D. M. A. Martin, D. P. O'Brien & E. G. D. Tuddenham

  3. Department of Biochemistry, The Medical School, Hugh Robson Building, George Square, Edinburgh, EH8 9XD, UK

    I. Polikarpov, A. Miller & C. W. G. Boys

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  8. E. G. D. Tuddenham
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  9. C. W. G. Boys
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Harlos, K., Martin, D., O'Brien, D. et al. Crystal structure of the extracellular region of human tissue factor. Nature 370, 662–666 (1994). https://doi.org/10.1038/370662a0

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