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Cloning and functional expression of a cyclic-nucleotide-gated channel from mammalian sperm

Nature volume 368, pages 859–863 (1994)Cite this article

Abstract

CYCLIC nucleotide-gated (CNG) channels serve as downstream targets of signalling pathways in vertebrate photoreceptor cells and olfactory sensory neurons (see ref. 1 for review). Ca2+ ions that enter through CNG channels2–5 intimately control these signalling pathways by regulating synthesis6 or hydrolysis7 of cyclic nucleotides, and by decreasing ligand sensitivity of CNG channels8. Several lines of evidence suggest that cyclic nucleotides and Ca2+ play important roles in chemotaxis of invertebrate sperm and fertilization (see ref. 9 for review), whereas their mechanisms of action in vertebrate sperm are largely unknown. Here we report the cloning and functional expression of a novel CNG channel from bovine testis. The channel polypeptide was functionally localized in sperm, but is also specifically expressed in cone photoreceptor cells. These channels might be involved in chemotaxis of sperm by controlling Ca2+ entry through a cyclic-nucleotide signalling pathway.

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Author notes

  1. Hans Hatt: Physiologisches Institut, Ruhr–Universität Bochum, Postfach 102148, 44780 Bochum, Germany

Authors and Affiliations

  1. Institut für Biologische Informationsverarbeitung, Forschungszentrum Jülich, Postfach 1913, 52425, Jülich, Germany

    Ingo Weyand, Matthias Godde, Stephan Frings, Jocelyn Weiner, Frank Müller, Wolfram Altenhofen, Hans Hatt & U. Benjamin Kaupp

Authors
  1. Ingo Weyand
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  2. Matthias Godde
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  3. Stephan Frings
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  4. Jocelyn Weiner
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  5. Frank Müller
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  6. Wolfram Altenhofen
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  7. Hans Hatt
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  8. U. Benjamin Kaupp
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Weyand, I., Godde, M., Frings, S. et al. Cloning and functional expression of a cyclic-nucleotide-gated channel from mammalian sperm. Nature 368, 859–863 (1994). https://doi.org/10.1038/368859a0

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