Abstract
THE development of mechanical force in skeletal muscle fibres is brought about by rapid increases in the intracellular calcium concentration (Ca2+transients) which can be detected by optical methods1–7. Local stimulation experiments8 and ultrastructural evidence9,10suggest that, at a microscopic level, these Ca2+ transients are generated by the release of Ca2+ ions from the terminal cisternae of the sarcoplasmic reticulum in response to the depolarization of the transverse tubules (t-tubules)11–14. Nevertheless, to date, there is no functional information on the exact location at which Ca2+ release takes place. The present experiments were designed to obtain direct evidence about dynamic changes in localization and microscopic distribution of Ca2+ in a single sarcomere using two independent novel methodologies: confocal spot detection of Ca2+ transients15,16 and Ca2+ imaging with pulsed laser excitation17,18.
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References
Ebashi, S. & Endo, M. Prog. Biophys. molec. Biol. 18, 123–183 (1968).
Sandow, A. A. Rev. Physiol. 32, 87–138 (1970).
Miledi, R., Parker, S. & Schalow, G. Prog. R. Soc. B198, 201–211 (1977).
Palade, P. & Vergara, J. J. gen. Physiol. 79, 679–707 (1982).
Baylor, S. M., Chandler, W. K. & Marshall, M. J. Physiol., Lond. 331, 179–210 (1982).
Vergara, J., Di Franco, M., Compagnon, D. & Suarez-lsla, B. Biophys. J. 59, 12–24 (1991).
Vergara, J. & Di Franco, M. Adv. Exp. Med. Biol. 311, 227–236 (1992).
Huxley, A. F. & Taylor, R. E. J. Physiol., Lond. 144, 426–441 (1958).
Winegrad, S. J. gen. Physiol. 55, 77–88 (1970).
Somlyo, A. V. et al. J. Cell Biol. 90, 577–594 (1981).
Endo, M. Physiol. Rev. 57, 71–108 (1977).
Schneider, M. F. & Chandler, W. K. Nature 242, 244–246 (1973).
Rios, E. & Pizarro, G. Physiol. Rev. 71, 849–908 (1991).
Fleisher, S. & Inui, M. A. Rev. Biophys. biophys. Chem 18, 333–364 (1989).
Escobar, A. & Vergara, J. Biophys. J. 64, A241 (1993).
Parker, I. & Ivorra, I. J. Physiol., Lond. 461, 133–165 (1992).
Hibino, M., Sigemori, M., Itoh, H., Nagayama, K. & Kinosita, K. Jr, Biophys. J. 59, 209–220 (1991).
Monck, J. R., Escobar, A., Robinson, I. M., Vergara, J. & Fernandez, J. M. Biophys. J. 66, A351 (1994).
Peachey, L. D. J. Cell Biol. 25, 209–231 (1965).
Vergara, J. & Escobar, A. Biophys. J. 64, A37 (1993).
Minta, A. et al. J. biol. Chem. 264, 8171–8178 (1989).
Wilson, T. & Carlini, A. R. Opt. Lett. 12, 227–229 (1987).
Dulhunty, A., Junankar, P. & Stanhope, C. Proc. R. Soc. B247, 69–75 (1992).
Takamatsu, T. & Wier, W. G. Cell Calcium 11, 111–120 (1990).
Niggli, E. & Lederer, W. J. Cell Calcium 11, 121–1130 (1990).
Parker, I. & Yao, Y. Proc. R. Soc. B246, 269–274 (1991).
Girard, S. & Clapham, D. Science 260, 229–232 (1993).
Grinvald, A., Frostig, R., Lieck, E. & Hildesheim, R. Physiol. Rev. 68, 1285–1358 (1988).
Monck, J., Oberhauser, A., Keating, T. & Fernandez, J. J. Cell Biol. 116, 745–759 (1992).
Sheppard, J. R. J. Microsc. 149, 73–75 (1998).
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Escobar, A., Monck, J., Fernandez, J. et al. Localization of the site of Ca2 + release at the level of a single sarcomere in skeletal muscle fibres. Nature 367, 739–741 (1994). https://doi.org/10.1038/367739a0
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DOI: https://doi.org/10.1038/367739a0
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