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Insight into E-selectin/ligand interaction from the crystal structure and mutagenesis of the lec/EGF domains

Abstract

The three-dimensional structure of the ligand-binding region of human E-selectin has been determined at 2.0 Å resolution. The structure reveals limited contact between the two domains and a coordination of Ca2+ not predicted from other C-type lectins. Structure/function analysis indicates a defined region and specific amino-acid side chains that may be involved in ligand binding. These features of the E-selectin/ligand interaction have important implications for understanding the recruitment of leukocytes to sites of inflammation.

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Graves, B., Crowther, R., Chandran, C. et al. Insight into E-selectin/ligand interaction from the crystal structure and mutagenesis of the lec/EGF domains. Nature 367, 532–538 (1994). https://doi.org/10.1038/367532a0

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