Letter | Published:

Transposition of a group II intron

Naturevolume 366pages176178 (1993) | Download Citation

Subjects

Abstract

AMONG mobile genetic elements, self-splicing introns are of particular interest. They belong to either group I or group II depending on their three-dimensional structure1& ndash;3. Homing, the systematic intron invasion of an intronless gene when it encounters its homologous intron-bearing allele, is the only means for intron mobility so far demonstrated4. It depends on the activity of the intron-encoded protein and is very specific for the acceptor site4& ndash;7. Intron transposition, the transfer of an intron to a novel site, predicted on the basis of phylogenetic studies8,9 and in vitro reverse-splicing experiments, has been proposed to be responsible for evolutionary intron spreading2,10& ndash;14. Here we present results from polymerase chain reaction experiments consistent with transposition of a group II intron. This event is proposed to account for the site-specific deletion in the mitochondria! chromosome of the fungus Podospora anserina that is associated with the premature death syndrome15 and might also be involved in the senescence process16 affecting this species.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Michel, F. & Dujon, B. EMBO J. 2, 33–38 (1983).

  2. 2

    Cech, R. R. A. Rev. Biochem. 59, 543–568 (1990).

  3. 3

    Michel, F., Umesono, K. & Ozeki, H. Gene 82, 5–30 (1989).

  4. 4

    Dujon, B. Gene 82, 91–114 (1989).

  5. 5

    Jacquier, A. & Dujon, B. Cell 41, 383–394 (1985).

  6. 6

    Macreadie, I. G., Scott, R. M., Zinn, A. R. & Butow, R. A. Cell 41, 395–402 (1985).

  7. 7

    Meunier, B., Tian, G. L., Macadre, C., Slonimski, P. P. & Lazowska, J. in Structure, Function and Biogenesis of Energy Transfer Systems (eds Quagliariello, E., Papa, S., Palmieri, F. & Saccone, C.) 169–174 (Elsevier, Amsterdam, 1990).

  8. 8

    Dujon, B., Colleaux, L., Jacquier, A., Michel, F. & Monteilhet, C. in Extrachromosomal Elements in Lower Eukaryotes (eds Wickner, R. B., Hinnebusch, A., Lambowitz, A. M., Gunsalus, I. C. & Hollaender, A.) 5–27 (Plenum, New York and London, 1986).

  9. 9

    Michel, F. & Cummings, D. J. Curr. Genet. 10, 69–79 (1985).

  10. 10

    Sharp, P. A. Cell 42, 397–400 (1985).

  11. 11

    Cech, T. R. Int. Rev. Cytol. 93, 3–22 (1985).

  12. 12

    Lambowitz, A. M. Cell 56, 323–326 (1989).

  13. 13

    Woodson, S. A. & Cech, T. R. Cell 57, 335–345 (1989).

  14. 14

    Möri, M. & Schmelzer, C. Cell 60, 629–636 (1990).

  15. 15

    Belcour, L., Begel, O. & Picard, M. Proc. natn. Acad. Sci. U.S.A. 88, 3579–3583 (1991).

  16. 16

    Rizet, G. C. r. Acad. Sci. 237, 838–840 (1953).

  17. 17

    Osiewacz, H. D. & Esser, K. Curr. Genet. 8, 299–305 (1984).

  18. 18

    Wright, R. M., Horrum, M. A. & Cummings, D. J. Cell 29, 505–515 (1982).

  19. 19

    Belcour, L., Begel, O., Mossé, M-O. & Vierny, C. Curr. Genet. 3, 13–21 (1981).

  20. 20

    Kück, U., Stahl, U. & Esser, K. Curr. Genet. 3, 151–156 (1981).

  21. 21

    Schmidt, U., Riederer, B., Mörl, M., Schmeizer, C. & Stahl, U. EMBO J. 9, 2289–2298 (1990).

  22. 22

    Sainsard-Chanet, A. & Begel, O. Nucleic Acids Res. 18, 779–783 (1990).

  23. 23

    Belcour, L. & Begel, O. Molec. gen. Genet. 153, 11–21 (1977).

  24. 24

    Jacquier, A. & Michel, F. Cell 50, 17–29 (1987).

  25. 25

    Augustin, S., Müller, M. W. & Schweyen, R. J. Nature 343, 383–386 (1990).

  26. 26

    Wright, R. M. & Cummings, D. J. Curr. Genet. 7, 457–464 (1983).

  27. 27

    Saisart-Chanet, A., Begel, O. & Belcour, L. J. molec. Biol. (in the press).

  28. 28

    Jacquier, A. Trends Biochem. Sci. 15, 351–354 (1990).

  29. 29

    Sharp, P. A. Science 254, 663 (1991).

  30. 30

    Cummings, D. J., McNally, K. L., Domenico, J. M. & Matsuura, E. T. Curr. Genet. 17, 375–402 (1990).

Download references

Author information

Affiliations

  1. Centre de G& eacute;n& eacute;tique Mol& eacute;culaire CNRS, 91198, Gif-sur-Yvette cedex, France

    • Carole H. Sellem
    • , Ga& euml;l Lecellier
    •  & L& eacute;on Belcour

Authors

  1. Search for Carole H. Sellem in:

  2. Search for Ga& euml;l Lecellier in:

  3. Search for L& eacute;on Belcour in:

About this article

Publication history

Received

Accepted

Issue Date

DOI

https://doi.org/10.1038/366176a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.