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Long-term correction of rat model of Parkinson's disease by gene therapy

Nature volume 362, pages 450–453 (1993)Cite this article

A Retraction to this article was published on 25 April 1996

Abstract

THE implantation of cells genetically modified to express tyrosine hydroxylase has been proposed for the treatment of Parkinson's disease1. Tyrosine hydroxylase converts tyrosine to L-DOPA and endogenous decarboxylase activity then converts L-DOPA to the neurotransmitter dopamine, which alleviates the symptoms of Parkinson's disease. Immortalized cells have been successfully used as intracerebral vehicles for transgene expression of tyrosine hydroxylase, but the tumorigenic potential of these cells prevents their application in humans1–4. Intracerebral expression of this enzyme has also been achieved using primary cells like skin fibroblasts5–7, but the ameliorating effect on a rat model for Parkinson's disease lasted for only a few weeks. We have found that co-transplantation of cultured myoblasts and myotubes enabled reporter genes to be expressed intracerebrally at high and stable levels8–10. Here we show that the intracerebral transplantation of plasmid-transfected primary muscle cells can substantially reduce for the long-term the asymmetric rotational behaviour in the rat model.

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Author notes

  1. Jon A. Wolff: To whom correspondence should be addressed.

Authors and Affiliations

  1. Departments of Pediatrics and Medical Genetics, Waisman Center, University of Wisconsin, Madison, Wisconsin, 53705, USA

    Shoushu Jiao, Vladimir Gurevich & Jon A. Wolff

Authors
  1. Shoushu Jiao
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  2. Vladimir Gurevich
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  3. Jon A. Wolff
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Jiao, S., Gurevich, V. & Wolff, J. Long-term correction of rat model of Parkinson's disease by gene therapy. Nature 362, 450–453 (1993). https://doi.org/10.1038/362450a0

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