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A new component of the transcription factor DRTF1/E2F

Nature volume 362, pages 83–87 (1993)Cite this article

A Correction to this article was published on 30 September 1993

Abstract

TRANSCRIPTION factor DRTF1/E2F coordinates events in the cell cycle with transcription by its cyclical interactions with important regulators of cellular proliferation like the retinoblastoma tumour-suppressor gene product (Rb) and the Rb-related protein, p107 (refs 1–8). DRTF1/E2F binding sites occur in the control regions of genes involved in proliferation9,10, and both Rb and p107 repress the capacity of DRTF1/E2F to activate transcription (refs 11, 12; M. Zamanian and N.B.L.T., manuscript submitted). Mutant Rb proteins isolated from tumour cells are unable to bind DRTF1/E2F (refs 11–13), and certain viral oncoproteins, such as adenovirus El A, sequester Rb and p107 in order to free active DRTF1/E2F (refs 5, 11, 12, 14, 15). Here we report the isolation of a complementary DNA encoding DRTF 1-polypeptide-1 (DP-1), a major sequence-specific binding protein that is present in DRTF1/E2F, including Rb- and p107-associated DRTF1/E2F. The DNA-binding domain of DP-1 contains a region that resembles that of E2F-1 (refs 16, 17), and recognizes the same sequence. DRTF1/E2F thus appears to contain at least two sequence-specific DNA-binding proteins.

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Author notes

  1. Janet F. Partridge

    Present address: Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, Washington, 98104, USA

  2. Nicholas F. Totty and J. Justin Hsuan: Ludwig Institute for Cancer Research, Middlesex Hospital/University College Branch, Courtauld Building, 91 Riding Street, London W1P 8BT, UK

  3. Nicholas B. La Thangue: To whom correspondence should be addressed.

Authors and Affiliations

  1. Laboratory of Eukaryotic Molecular Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK

    Rowena Girling, Janet F. Partridge, Lasantha R. Bandara, Neil Burden, Nicholas F. Totty, J. Justin Hsuan & Nicholas B. La Thangue

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Girling, R., Partridge, J., Bandara, L. et al. A new component of the transcription factor DRTF1/E2F. Nature 362, 83–87 (1993). https://doi.org/10.1038/362083a0

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