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Drug assay using antibody mimics made by molecular imprinting

Nature volume 361, pages 645–647 (1993)Cite this article

Abstract

LIGAND-BINDING assays are used for determination of minute amounts of substances in the bloodstream. Such assays require a receptor that specifically binds the substance of interest. The receptor used is often an antibody1–5, but antibodies require special handling and a costly production procedure5. We have used molecular imprinting, a method for creating selective recognition sites in synthetic polymers6–8, to prepare polymers that mimic antibody combining sites. Molecular imprints made against theophylline9 and diazepam10 showed strong binding and cross-reactivity profiles similar to those of antibodies. Here we describe a new radiolabelled ligand-binding assay, the molecularly im-printed sorbent assay, which uses antibody mimics. This assay accurately measures drug levels in human serum, with results comparable to those obtained using a well established immunoassay technique. Antibody mimics, which are stable and readily prepared by molecular imprinting, may provide a useful general alternative to antibodies.

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References

  1. Yalow, R. S. & Berson, S. A. Nature 184, 1648–1649 (1959).

    Article  CAS  Google Scholar 

  2. Köhler, G. & Milstein, C. Nature 256, 495–497 (1975).

    Article  ADS  Google Scholar 

  3. Oellerich, M. J. clin. Chem. clin. Biochem. 22, 895–904 (1984).

    CAS  PubMed  Google Scholar 

  4. Gosling, J. P. Clin. Chem. 36, 1408–1427 (1990).

    CAS  PubMed  Google Scholar 

  5. Kurstak, E. in Enzyme Immunodiagnosis (ed. Kurstak, E.) 5–11 (Academic, London, 1986).

    Book  Google Scholar 

  6. Sellergren, B., Ekberg, B. & Mosbach, K. J. Chromatogr. 347, 1–10 (1985).

    Article  CAS  Google Scholar 

  7. Wulff, G. Am. chem. Soc. Symp. Series 308, 186–230 (1986).

    CAS  Google Scholar 

  8. Shea, K. J. & Sasaki, D. Y. J. Am. chem. Soc. 113, 4109–4120 (1991).

    Article  CAS  Google Scholar 

  9. Hendeles, L., Weinberger, M. & Johnson, G. Clin. Pharmacokinet. 3, 294–312 (1978).

    Article  CAS  Google Scholar 

  10. Harvey, S. L. in The Pharmacological Basis of Therapeutics (eds Gilman, A. G., Goodman, L. S., Rall, T. W. & Murad, F.) 339–351 (Dekker, New York, 1985).

    Google Scholar 

  11. Ekberg, B. & Mosbach, K. Trends Biotechnol. 7, 92–96 (1989).

    Article  CAS  Google Scholar 

  12. Sellergren, B., Lepistö, M. & Mosbach, K. J. Am. chem. Soc. 110, 5853–5860 (1988).

    Article  CAS  Google Scholar 

  13. O'Shannessy, D. J., Ekberg, B., Andersson, L. I. & Mosbach, K. J. Chromatogr. 470, 391–399 (1989).

    Article  CAS  Google Scholar 

  14. Andersson, L. I. & Mosbach, K. J. Chromatogr. 516, 313–322 (1990).

    Article  CAS  Google Scholar 

  15. Wulff, G. & Minarik, M. J. Iiq. Chromatogr. 13, 2987–3000 (1990).

    CAS  Google Scholar 

  16. Fischer, L., Müller, R., Ekberg, B. & Mosbach, K. J. Am. chem. Soc. 113, 9358–9360 (1991).

    Article  CAS  Google Scholar 

  17. Andersson, L. I. thesis, Lund Univ. (1991).

  18. Meffin, P. J. & Miners, J. O. in Progress in Drug Metabolism Vol. 4 (eds Bridges, J. W. & Chasseaud, L. F.) 261–307 (Wiley, London, 1980).

    Google Scholar 

  19. Peng, G. W., Gadalla, M. A. F. & Chiou, W. L. Clin. Chem. 24, 357–361 (1978).

    CAS  PubMed  Google Scholar 

  20. Mura, P., Piriou, A., Fraillon, P., Papet, Y. & Reiss, D. J. Chromatogr. 416, 303–310 (1987).

    Article  CAS  Google Scholar 

  21. Castro, A., Ibanez, J., Voight, W., Noto, T. & Malkus, H. Clin. Chem. 24, 944–946 (1978).

    CAS  PubMed  Google Scholar 

  22. Chang, J., Gotcher, S. & Gushaw, J. B. Clin. Chem. 28, 361–367 (1982).

    CAS  PubMed  Google Scholar 

  23. Poncelet, S. M. et al. J. Immunoassay, 11, 77–88 (1990).

    Article  CAS  Google Scholar 

  24. Baselt, R. C. in Advances in Analytical Toxicology Vol. 1 (ed. Baselt, R. C.) 81–123 (Biomedical, Foster City, CA, 1984).

    Google Scholar 

  25. Altunkaya, D. & Smith, R. N. Forensic Sci. Int. 39, 23–37 (1988).

    Article  CAS  Google Scholar 

  26. Dietzler, D. N. et al. Clin. chim. Acta 101, 163–181 (1980).

    Article  CAS  Google Scholar 

  27. Cram, D. J. Nature 356, 29–36 (1992).

    Article  ADS  CAS  Google Scholar 

  28. Rebek, J. Jr. Angew. chem. int. Ed. engl. 29, 245–255 (1990).

    Article  Google Scholar 

  29. Deslongchamps, G., Galán, A., de Mendoza, J. & Rebek, J. Jr Angew. chem. int. Ed. Engl. 31, 61–63 (1992).

    Article  Google Scholar 

  30. Tijssen, P. Laboratory Techniques in Biochemistry and Molecular Biology, Practice and Theory of Enzyme Immunoassays 5th printing (Elsevier, Amsterdam, 1988).

    Google Scholar 

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Author information

Author notes

  1. George Vlatakis

    Present address: Foundation for Research and Technology, Institute of Molecular Biology and Biotechnology, 711 10 Heraklion, Crete, Greece

  2. Ralf Müller

    Present address: Johnson & Johnson Medical, Ostrasse 1, 2000, Norderstedt, Germany

Authors and Affiliations

  1. Department of Pure and Applied Biochemistry, Chemical Centre, University of Lund, PO Box 124, S-221 00, Lund, Sweden

    George Vlatakis, Lars I. Andersson, Ralf Müller & Klaus Mosbach

Authors
  1. George Vlatakis
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  2. Lars I. Andersson
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  3. Ralf Müller
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  4. Klaus Mosbach
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Vlatakis, G., Andersson, L., Müller, R. et al. Drug assay using antibody mimics made by molecular imprinting. Nature 361, 645–647 (1993). https://doi.org/10.1038/361645a0

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