Abstract
HAEMATOPOIETIC stem cells are a population of cells capable both of self renewal and of differentiation into a variety of haematopoietic lineages1–4. Enrichment techniques of human haematopoietic stem cells have used the expression of CD34, present on bone marrow progenitor cells5–7. But most CD34+ bone marrow cells are committed to their lineage, and more recent efforts have focused on the precise characterization of the pluripotent subset of CD34+ cells8–17. Here we report the characterization of two distinct subsets of pluripotent stem cells from human fetal bone marrow, a CD34+, HLA-DR+, CD38− subset that can differentiate into all haematopoietic lineages, and a distinct more primitive subset, that is CD34+, HLA-DR−, CD38−, that can differentiate into haematopoietic precursors and stromal cells capable of supporting the differentiation of these precursors. These data represent, to our knowledge, the first identification of a single cell capable of reconstituting the haematopoietic cells and their associated bone marrow microenvironment.
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This article has been retracted. Please see the retraction notice for more detail: https://doi.org/10.1038/368664a0
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Huang, S., Terstappen, L. RETRACTED ARTICLE: Formation of haematopoietic microenvironment and haematopoietic stem cells from single human bone marrow stem cells. Nature 360, 745–749 (1992). https://doi.org/10.1038/360745a0
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DOI: https://doi.org/10.1038/360745a0
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