Competitive reverse transcription-polymerase chain reaction (RT-PCR) can be used to obtain quantitative information of mRNA levels comparable to traditional RNA blot techniques, with the added advantages of PCR.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Enhancement of PCR Sensitivity and Yield Using Thiol-modified Primers
Scientific Reports Open Access 05 October 2018
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Get just this article for as long as you need it
$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Larrick, J. W. Trends Biotechnology 10, 146–152 (1992).
Chelly, J., Kaplan, J.-C., Gautron, S. & Kahn, A. Nature 333, 858–860 (1988).
Kawasaki, E. S. in PCR Protocols: A Guide to Methods and Applications (eds Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. J.) 21–27 (Academic, San Diego, California, 1991).
Rappolee, D. A., Mark, D., Banda, M. J. & Werb, Z. Science 241, 708–712 (1991).
Brenner, C. A. et al. BioTechniques 7, 1096–1103 (1989).
Mocharla, H., Mocharla, H. & Hodes, M. E. Gene 91, 271–275 (1990).
Byrne, B. C., Li, J. J., Sninsky, J. & Poiesz, B. J. Nucleic Acids Res. 16, 4165–4170 (1988).
Wang, A. M., Doyle, M. V. & Mark, D. F. Proc. natn. Acad. Sci. U.S.A. 86, 9717–9721 (1989).
Schmidt, E. E. & Merrill, G. F. Molec. cell. Biol. 11, 3726–3734 (1991).
Siebert, P. D. & Fukuda, M. J. biol. Chem. 260, 3868–3874 (1985).
Elder, P. K., French, C. L., Subramaniam, M., Schmidt, L. J. & Getz, M. J. Molec. cell. Biol. 8, 480–485 (1988).
Murphy, L. D., Herzog, D. E., Rudick, J. B., Fojo, A. T. & Bates, S. E. Biochemistry 29, 10351–10356 (1990).
Becker-André, M. & Hahlbrock, K. Nucleic Acids Res. 17, 9437–9446 (1989).
Gilliand, G., Perrin, S., Blanchard, K. & Bunn, H. F. Proc. natn. Acad. Sci. U.S.A. 87, 2725–2729 (1990).
Uberla, K., Platzer, C., Diamantstein, T. & Blankenstein, T. PCR Methods and Applications 1, 136–139 (1991).
Siebert, P. D. & Larrick, J. W. BioTechniques (in the press).
Lyons, C. R., Orloff, G. J. & Cunningham, J. M. J. biol. Chem. 267, 6370–6374 (1992).
Xie, Q. et al. Science 256, 225–228 (1992).
Lowenstein, C. J., Glatt, C. S., Bredt, D. S. & Snyder, S. H. Proc. natn. Acad. Sci. U.S.A. 89, 6711–6715 (1992).
Nedelman, J., Heagerty, P. & Lawrence, E. CABIOS 8, 65–70 (1992).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Siebert, P., Larrick, J. Competitive PCR. Nature 359, 557–558 (1992). https://doi.org/10.1038/359557a0
Issue Date:
DOI: https://doi.org/10.1038/359557a0
This article is cited by
-
Role of green innovation, trade and energy to promote green economic growth: a case of South Asian Nations
Environmental Science and Pollution Research (2022)
-
Enhancement of PCR Sensitivity and Yield Using Thiol-modified Primers
Scientific Reports (2018)
-
A multiplex sensitive quantification of microRNAs based on competitive PCR
Biotechnology and Bioprocess Engineering (2017)
-
Quantification of constitutive endothelial and inducible nitric oxide synthase mRNA by competitive reverse transcription-polymerase chain reaction
International Journal of Angiology (2011)
-
Implementation of an artificial reaction control in a TaqMan method for PCR detection of Ralstonia solanacearum race 3 biovar 2
European Journal of Plant Pathology (2009)