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T-complex polypeptide-1 is a subunit of a heteromeric particle in the eukaryotic cytosol

Nature volume 358, pages 249–252 (1992)Cite this article

Abstract

THE murine t-complex encodes t-complex polypeptide-1 (TCP1)1, which is constitutively expressed in almost all cells, and upregulated during spermatogenesis2,3. Mammalian sequences have >96% identity with each other, and >60% identity with Drosophila melanogaster and yeast orthologues4–9. TCP1 is essential in yeast9, and is postulated to be the cytosolic mammalian equivalent of groEL10,11. We report here that, in the native state, murine and human TCP1 is distributed throughout the cytosol as an 800K–950K hetero-oligomeric particle in association with four to six unidentified proteins and two Hsp70 heat-shock proteins. Negative-stain electron microscopy indicates that the structure is two stacked rings, 12–16 nm in diameter. Therefore, despite similarities with the chaperonin 60 proteins, these data indicate that TCP1 is biochemically and structurally unique. We suggest that TCP1 may represent one of a family of molecules in the eukaryotic cytosol involved in protein folding and regulated in part by their heteromeric associations.

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References

  1. Silver, L. M., Artzt, K. & Bennett, D. Cell 17, 275–284 (1979).

    Article  CAS  Google Scholar 

  2. Willison, K. et al. Cell 57, 621–632 (1989).

    Article  CAS  Google Scholar 

  3. Willison, K. R., Hynes, G., Davies, P., Goldsborough, A. & Lewis, V. A. Genet. Res. 56, 193–201 (1990).

    Article  CAS  Google Scholar 

  4. Kirchhoff, C. & Willison, K. Nucleic Acids Res. 18, 4248 (1990).

    Article  Google Scholar 

  5. Willison, K. R., Dudley, K. & Potter, J. Cell 44, 727–738 (1986).

    Article  CAS  Google Scholar 

  6. Ahmad, S. & Gupta, R. S. Biochim. biophys. Acta 1089, 253–255 (1990).

    Article  Google Scholar 

  7. Morita, T., Kubota, H., Gachelin, G., Nozaki, M. & Matsushiro, A. Biochim. biophys. Acta 1129, 96–99 (1991).

    Article  CAS  Google Scholar 

  8. Ursic, D. & Ganetsky, B. Gene 68, 267–274 (1988).

    Article  CAS  Google Scholar 

  9. Ursic, D. & Culbertson, M. R. Molec. cell. Biol. 11, 2629–2640 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Gupta, R. S. Biochem. Int. 4, 833–839 (1990).

    Google Scholar 

  11. Ellis, R. J. Science 250, 954–959 (1990).

    Article  ADS  CAS  Google Scholar 

  12. Linquist, S. & Craig, E. A. Rev. Genet. 22, 631–677 (1988).

    Article  Google Scholar 

  13. Maekawa, M. O'Brien, D. A., Allen, R. L. & Eddy, E. M. Biol. Repro. 40, 843–852 (1989).

    Article  CAS  Google Scholar 

  14. Hendrix, R. W. J. molec. Biol. 129, 375–392 (1979).

    Article  CAS  Google Scholar 

  15. Hutchinson, E. G. et al. EMB0 J. 8, 1485–1490 (1989).

    Article  CAS  Google Scholar 

  16. Phipps, B. M., Hoffmann, A., Stetter, K. O. & Baumeister, W. EMBO J. 10, 1711–1722 (1991).

    Article  CAS  Google Scholar 

  17. Trent, J. D., Nimmesgern, E., Wall, J. S., Hartl, F.-U. & Horwich, A. L. Nature 354, 490–493 (1991).

    Article  ADS  CAS  Google Scholar 

  18. Dahlmann, B. et al. FEBS Lett. 251, 125–131 (1989).

    Article  CAS  Google Scholar 

  19. Hanks, S. K., Quinn, A. M. & Hunter, T. Science 241, 42–52 (1988).

    Article  ADS  CAS  Google Scholar 

  20. Ellis, R. J. & van der Vies, S. M. A. Rev. Biochem. 60, 321–347 (1991).

    Article  CAS  Google Scholar 

  21. Yaffe, M. B. et al. Nature 358, 245–248 (1992).

    Article  ADS  CAS  Google Scholar 

  22. Kim, Y. S., Perdomo, J. & Nordberg, J. J. biol. Chem. 246, 5466–5476 (1971).

    Article  CAS  Google Scholar 

  23. Corbett, J. & Dunn, M. J. Methods in Molecular Biology: Membrane Methods (eds Graham, J. & Higgins, J.) (Humana, New Jersey, in the press).

  24. Mehra, V., Sweetser, D. & Young, R. A. Proc. natn. Acad. Sci. U.S.A. 83, 7013–7017 (1986).

    Article  ADS  CAS  Google Scholar 

  25. Hemmingsen, S. M. et al. Nature 333, 330–334 (1988).

    Article  ADS  CAS  Google Scholar 

  26. Waterston, R. et al. Nature Genet. 1, 114–123 (1992).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Cancer Research, Chester Beatty Laboratories, Fulham Road, London, SW3 6JB, UK

    Victoria A. Lewis, Gillian M. Hynes, Dong Zheng, Helen Saibil & Keith Willison

  2. Department of Crystallography, Birkbeck College, London, WC1E 7HX, UK

    Dong Zheng & Helen Saibil

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  1. Victoria A. Lewis
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  2. Gillian M. Hynes
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  3. Dong Zheng
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  5. Keith Willison
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Lewis, V., Hynes, G., Zheng, D. et al. T-complex polypeptide-1 is a subunit of a heteromeric particle in the eukaryotic cytosol. Nature 358, 249–252 (1992). https://doi.org/10.1038/358249a0

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