Oligonucleotide-immobilized plastic plates provide a rapid and easy-to-use tool for use in mRNA research, including the long-term storage, amplification and detection of the specific message, in vitro synthesis of both strands of sense and antisense mRNA, and ligation of cDNA to other DNA molecules.
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References
Sambrook, J., Fritsch, E. F. & Maniatis, T. in Molecular Cloning, A Laboratory Manual: Extraction, Purification and Analysis of Messenger RNA from Eukaryotic Cells 2nd Edn 7.1–7.87 (Cold Spring Harbor, New York, 1989).
Alwine, J. C., Kemp, D. J. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).
Melton, D. A. et al. Nucleic Acids Res. 7, 1175–1193 (1984).
Kawasaki, E. S. & Wang, A. M. in PCR Technology: Detection of Gene Expression (ed. Erlich, E. A.) 89–97 (Stockton, New York, 1989).
White, B. A. & Bancroft, F. C. J. biol. Chem. 257, 8569–8572 (1982).
Huynh, T. V., Young, R. A. & Davis, R. W. in DNA Cloning: A Practical Approach — Constructing and Screening cDNA Libraries in λgylO and λgtll (ed. Glover, D. M.) Vol. 1, 49 (IRL, Oxford, 1985).
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Mitsuhashi, M., Keller, C. & Akitaya, T. Gene manipulation on plastic plates. Nature 357, 519–520 (1992). https://doi.org/10.1038/357519a0
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DOI: https://doi.org/10.1038/357519a0
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