Abstract
CYCLOBUTANE pyrimidine dimers (CPDs) are the predominant product of photodamage in DNA after exposure of cells to ultraviolet light1,2 and are cytotoxic, mutagenic and carcinogenic in a variety of cellular and animal systems3–5. In prokaryotes, enzymes and protein complexes have been characterized that remove or reverse CPDs in DNA6–8. Micrococcus luteus and T4 phage-infected Escherichia coli contain a specific N-glyco-sylase/apurinic-apyrimidinic lyase that catalyses a two-step DNA incision process at sites of CPDs, thus initiating base excision repair of these lesions7,9–2. It is well established that CPDs are recognized and removed from eukaryotic DNA by excision repair processes but very little information exists concerning the nature of the proteins involved in CPD recognition and DNA incision events7,12,13. We report here that an enzyme functionally similar to the prokaryotic N-glycosylase/apurinic–apyrimidinic lyases exists in Saccharomyces cerevisiae. To our knowledge, this is the first time such an activity has been found in a eukaryote and is also the first example of an organism having both direct reversal and base excision repair pathways for the removal of CPDs from DNA.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Patrick, M. H. & Gray, D. Photochem. Photobiol. 24, 507–513 (1976).
Patrick, M. H. Photochem. Photobiol. 25, 357–372 (1977).
Harm, W. The Biological Effects of Ultraviolet Radiation (Cambridge Univ. Press, 1980).
Coohill, T. P. Photochem. Photobiol. 46, 1043–1050 (1987).
Hart, R. W. & Setlow, R. B. Molecular Mechanisms of DNA Repair Part B 719–724 (Plenum. New York, 1975).
Sancar, A. & Sancar, G. B. A. Rev. Biochem. 57, 29–67 (1988).
Friedberg, E. C. DNA Repair (Freeman, New York, 1985).
Sancar, G. B. Mutat. Res. 236, 147–160 (1990).
Dodson, M. L. & Lloyd, R. S. Mutat. Res. 218, 49–65 (1990).
Haseltine, W. A. et al. Nature 285, 634–641 (1980).
Grafstrom, R. H., Park, L. & Grossman, L. J. biol. Chem. 257, 13465–13474 (1982).
Radany, E. H. & Friedberg, E. C. J. Virol. 41, 88–96 (1982).
Friedberg, E. C. et al. J. supramolec. Struct cell. Biochem. 16, 91–103 (1981).
Gossett, J., Lee, K., Cunningham, R. P. & Doetsch, P. W. Biochemistry 27, 2629–2634 (1988).
Sancar, A., Smith, F. W. & Sancar, G. B. J. biol. Chem. 259, 6028–6032 (1984).
Doetsch, P. W., Helland, D. E. & Haseltine, W. A. Biochemistry 25, 2212–2220 (1986).
Friedberg, E. C., Ganesan, A. K. & Seawall, P. C. Meth. Enzym. 65, 191–201 (1980).
Radany, E. H. & Friedberg, E. C. Nature 286, 182–185 (1980).
Seawall, P. C., Smith, C. A. & Ganesan, A. K. J. Virol. 35, 790–792.
Mazumder, A. et al. Am. chem. Soc. 111, 8029–8030 (1989).
Gordon, L. K. & Haseltine, W. A. J. biol. Chem. 255, 12047–12050 (1980).
Friedberg, E. C. Microbiol. Rev. 52, 70–102 (1988).
Sung, P., Prakash, L., Matson, S. W. & Prakash, S. Proc. natn. Acad. Sci. U.S.A. 84, 8951–8955 (1987).
Jentsch, S., McGrath, J. P. & Varshavsky, A. Nature 329, 131–134 (1987).
Weinert, T. A. & Hartwell, L. H. Science 241, 317–322 (1988).
Bekker, M. L. Kaboev, O. K., Akhmedov, A. T. & Luchkina, L. A. J. Bact. 142, 322–324 (1980).
Waldstein, E. A., Peller, S. & Setlow, R. B. Proc. natn. Acad. Sci. U.S.A. 76, 3746–3750 (1979).
Prakash, L. Mutat. Res. 45, 13–20 (1977).
Reynolds, R. J. & Friedberg, E. C. J. Bact. 146, 692–704 (1981).
Bankman, M., Prakash, L. & Prakash, S. Nature 355, 555–558 (1992).
Dowd, D. R. & Lloyd, R. S. J. biol. Chem. 265, 3424–3431 (1990).
Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).
Payne, G., Heelis, P. F., Rohrs, B. R. & Sancar, A. Biochemistry 26, 7121–7127 (1987).
Baily, V. & Verly, W. G. Biochem. J. 259, 761–768 (1989).
Hasegawa, S. L. et al. Nucleic Acids Res. 19, 4915–4920 (1991).
Levin, J. D. & Demple, B. J. biol. Chem. 263, 8066–8071 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hamilton, K., Kirn, P. & Doetsch, P. A eukaryotic DNA glycosylase/lyase recognizing ultraviolet light-induced pyrimidine dimers. Nature 356, 725–728 (1992). https://doi.org/10.1038/356725a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/356725a0
This article is cited by
-
UV-induced endonuclease III-sensitive sites at the mating type loci inSaccharomyces cerevisiae are repaired by nucleotide excision repair: RAD7 and RAD16 are not required for their removal fromHMLα
Molecular and General Genetics MGG (1996)
-
Selection of Arabidopsis cDNAs that partially correct phenotypes of Escherichia coli DNA-damage-sensitive mutants and analysis of two plant cDNAs that appear to express UV-specific dark repari activities
Plant Molecular Biology (1993)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
