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Primary structure of dystrophin-associated glycoproteins linking dystrophin to the extracellular matrix

Abstract

The primary sequence of two components of the dystrophin–glycoprotein complex has been established by complementary DNA cloning. The transmembrane 43K and extracellular 156K dystrophin-associated glycoproteins (DAGs) are encoded by a single messenger RNA and the extracellular 156K DAG binds laminin. Thus, the 156K DAG is a new laminin-binding glycoprotein which may provide a linkage between the sarcolemma and extracellular matrix. These results support the hypothesis that the dramatic reduction in the 156K DAG in Duchenne muscular dystrophy leads to a loss of a linkage between the sarcolemma and extra-cellular matrix and that this may render muscle fibres more susceptible to necrosis.

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References

  1. Hoffman, E. P., Brown, R. H. & Kunkel, L. M. Cell 51, 919–928 (1987).

    Article  CAS  Google Scholar 

  2. Koenig, M., Monaco, A. P. & Kunkel, L. M. Cell 53, 219–228 (1988).

    Article  CAS  Google Scholar 

  3. Arahata K. et al. Nature 333, 861–866 (1988).

    Article  ADS  CAS  Google Scholar 

  4. Hoffman, E. P. et al. N. Engl. J. Med. 318, 1363–1368 (1988).

    Article  CAS  Google Scholar 

  5. Watkins, S. C., Hoffman, E. P., Slayter, H. S. & Kunkel, L. M. Nature 333, 863–866 (1988).

    Article  ADS  CAS  Google Scholar 

  6. Cooper, B. J. et al. Nature 334, 154–156 (1988).

    Article  ADS  CAS  Google Scholar 

  7. Campbell, K. P. & Kahl, S. D. Nature 338, 259–262 (1989).

    Article  ADS  CAS  Google Scholar 

  8. Ervasti, J. M., Ohlendieck, K., Kahl, S. D., Gavet, M. G. & Campbell, K. P. Nature 345, 315–319 (1990).

    Article  ADS  CAS  Google Scholar 

  9. Ervasti, J. M. & Campbell, K. P. Cell 66, 1121–1131 (1991).

    Article  CAS  Google Scholar 

  10. Ohlendieck, K., Ervasti, J. M., Snook, J. B. & Campbell, K. P. J. Cell Biol. 112, 135–148 (1991).

    Article  CAS  Google Scholar 

  11. Ohlendieck, K. & Campbell, K. P. FEBS Lett. 283, 230–234 (1991).

    Article  CAS  Google Scholar 

  12. Ohlendieck, K. & Campbell, K. P. J. Cell Biol. 115, 1685–1694 (1991).

    Article  CAS  Google Scholar 

  13. Devi, L. FEBS Lett. 280, 189–194 (1991).

    Article  CAS  Google Scholar 

  14. Ehrig, K., Leivo, I., Argraves, W. S., Ruoslahti, E. & Engvall, E. Proc. natn. Acad Sci. U.S.A. 87, 3264–3268 (1990).

    Article  ADS  CAS  Google Scholar 

  15. Hunter, D. D., Shah, V., Merlie, J. P. & Sanes, J. R. Nature 338, 229–234 (1989).

    Article  ADS  CAS  Google Scholar 

  16. Sanes, J. R., Engvall, E., Butkowsky, R. & Hunter, D. D. J. Cell Biol. 111, 1685–1699 (1990).

    Article  CAS  Google Scholar 

  17. Hassell, J. R., Kimura, J. H. & Hascall, V. C. A. Rev. Biochem. 55, 539–567 (1986).

    Article  CAS  Google Scholar 

  18. Höök, M., Kjellén, L., Johansson, S. & Robinson, J. A. Rev. Biochem. 53, 847–869 (1984).

    Article  Google Scholar 

  19. Lesot, H., Kühl, U. & von der Mark, K. EMBO J. 2, 861–865 (1983).

    Article  CAS  Google Scholar 

  20. Clegg, D. O., Helder, J. C., Hann, B. C., Hall, D. E. & Reichardt, L. F. J. Cell Biol. 107, 699–7095 (1988).

    Article  CAS  Google Scholar 

  21. Mecham, R. P. FASEB J. 5, 2538–2546 (1991).

    Article  CAS  Google Scholar 

  22. Volk, T., Fessler, L. I. & Fessler, J. H. Cell 63, 525–536 (1990).

    Article  CAS  Google Scholar 

  23. Smalheiser, N. R. & Schwartz, N. B. Proc. natn. Acad. Sci. U.S.A. 84, 6457–6461 (1987).

    Article  ADS  CAS  Google Scholar 

  24. Menke, A. & Jockusch, M. Nature 349, 69–71 (1991).

    Article  ADS  CAS  Google Scholar 

  25. Franco, A. & Lansman, J. B. Nature 344, 670–673 (1990).

    Article  ADS  CAS  Google Scholar 

  26. Turner, P. R., Fong, P., Denetclaw, W. F. & Steinhardt, R. A. J. Cell Biol. 115, 1701–1712 (1991).

    Article  CAS  Google Scholar 

  27. Bonilla, E. & Moggio, E. Neurology 36, 171 (1986).

    Google Scholar 

  28. Carpenter, S. & Karpati, G. Brain 102, 147–161 (1979).

    Article  CAS  Google Scholar 

  29. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).

    Article  CAS  Google Scholar 

  30. Jay, S. D. et al. Science 248, 490–492 (1990).

    Article  ADS  CAS  Google Scholar 

  31. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  32. Smith, D. B. & Johnson, K. S. Gene 67, 31–40 (1988).

    Article  CAS  Google Scholar 

  33. Gorka, J., McCourt, D. W. & Schwartz, B. D. Peptide Res. 2, 1–5 (1989).

    Google Scholar 

  34. McPherson, P. S. et al. Neuron 7, 17–25 (1991).

    Article  CAS  Google Scholar 

  35. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  36. Campbell, K. P. et al. J. biol. Chem. 262, 6460–6463 (1987).

    CAS  PubMed  Google Scholar 

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Ibraghimov-Beskrovnaya, O., Ervasti, J., Leveille, C. et al. Primary structure of dystrophin-associated glycoproteins linking dystrophin to the extracellular matrix. Nature 355, 696–702 (1992). https://doi.org/10.1038/355696a0

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