Abstract
Poly(A) polymerase has a critical role in the synthesis of messenger RNA in eukaryotic cells. The isolation and characterization of complementary DNAs encoding bovine poly(A) polymerase is described here. The predicted sequences of the mRNA and protein reveal features that provide insights into how the enzyme functions and how it might be regulated. Poly(A) polymerase expressed from a cloned cDNA is fully functional in in vitro assays, and mutational analyses have identified a putative regulatory domain that enhances, but is not essential for, activity.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Edmonds, M. & Abrams, R. J. biol. Chem. 235, 1142–1149 (1960).
Edmonds, M. in The Enzymes Vol 15 (ed. Boyer, P. D.) 217–244 (Academic, New York, 1982).
Jacob, S. T. & Rose, K. M. in Enzymes of Nucleic Acid Synthesis and Modification Vol 2, (ed., Jacob, S. T.) 135–157 (CRC Press, Boca Raton, Florida, 1983).
Darnell, J. E., Wall, R. & Tushinski, R. J. Proc. natn. Acad. Sci. U.S.A. 68, 1321–1325 (1971).
Lee, S., Mendecki, J. & Brawerman, G. Proc. natn. Acad. Sci. U.S.A. 68, 1331–1335 (1971).
Edmonds, M., Vaughan, M. & Nakazato, H. Proc. natn. Acad. Sci. U.S.A. 68, 1336–1340 (1971).
Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).
Fitzgerald, M. & Shenk, T. Cell 24, 251–260 (1981).
Manley, J. L. Biochem. biophys. Acta 950, 1–12 (1988).
Humphrey, T. & Proudfoot, N. J. Trends Genet. 4, 243–245 (1988).
Manley, J. L. Cell 33, 595–605 (1983).
Manley, J. L., Yu, H. & Ryner, L. C. Molec. cell. Biol. 5, 373–379 (1985).
Moore, C. L. & Sharp, P. A. Cell 36, 581–591 (1984).
Moore, C. L. & Sharp, P. A. Cell 41, 845–855 (1985).
Wickens, M. Trends biochem. Sci. 15, 277–281 (1990).
Takagaki, Y., Ryner, L. C. & Manley, J. L. Cell 52, 731–742 (1988).
Christofori, G. & Keller, W. Cell 54, 875–889 (1988).
Takagaki, Y., Ryner, L. C. & Manley, J. L. Genes Dev. 3, 1711–1724 (1989).
Gilmartin, G. M. & Nevins, J. R. Genes Dev. 3, 2180–2189 (1989).
Takagaki, Y., Manley, J. L., MacDonald, C. C., Wilusz, J. & Shenk, T. Genes Dev. 4, 2112–2120 (1990).
Christofori, G. & Keller, W. Molec. cell. Biol. 9, 193–203 (1989).
Ryner, L. C., Takagaki, Y. & Manley, J. L. Molec. cell. Biol. 9, 4229–4238 (1989).
Bardwell, V. J., Zarkower, D., Edmonds, M. & Wickens, M. Molec. cell. Biol. 10, 846–849 (1990).
Wahle, E. J. biol. Chem. 266, 3131–3139 (1991).
Tsiapalis, C., Dorson, J. & Bollum, F. J. J. biol. Chem. 250, 4486–4496 (1975).
Kozak, M. J. Cell Biol. 108, 229–241 (1989).
Shaw, G. & Kamen, R. Cell 46, 659–667 (1986).
Jones, T. R. & Cole, M. D. Molec. cell. Biol. 7, 4513–4521 (1987).
Wilson, T. & Treisman, R. Nature 336, 396–399 (1988).
Caput, D. et al. Proc. natn. Acad. Sci. U.S.A. 83, 1670–1674 (1986).
Bandziulis, R. J., Swanson, M. S. & Dreyfuss, G. Genes Dev. 3, 431–437 (1989).
Query, C. C., Bentley, R. C. & Keene, J. D. Cell 57, 89–101 (1989).
Kenan, D. J., Query, C. C. & Keene, J. D. Trends biochem. Sci. 16, 214–220 (1991).
Adam, S. A., Nakagawa, T., Swanson, M. S., Woodruff, T. K. & Dreyfuss, G. Molec. cell. Biol. 6, 2932–2943 (1986).
Sachs, A. B., Bond, M. W. & Kornberg, R. D. Cell 45, 827–835 (1986).
Poch, O., Sauvaget, I., Delaure, M. & Tordo, N. EMBO J. 8, 3867–3874 (1989).
Lundblad, V. & Blackburn, E. H. Cell 60, 529–530 (1990).
Argos, P. Nucleic Acids Res. 16, 9909–9916 (1988).
Robbins, J., Dilworth, S. M., Laskey, R. A. & Dingwall, C. Cell 64, 615–624 (1991).
Langan, T. A. J. biol. Chem. 257, 14835–14846 (1982).
Shenoy, S. et al. Cell 57, 763–774 (1989).
Jackson, R. J. & Standart, N. Cell 62, 15–24 (1990).
Wickens, M. Trends biochem. Sci. 15, 320–324 (1990).
Kozak, M. Molec. cell. Biol. 8, 2737–2744 (1988).
Lee, C. et al. Analyt. Biochem. 166, 308–312 (1987).
Hager, D. & Burgess, R. Analyt. Biochem. 109, 76–86 (1980).
Aebersold, R. H., Leavitt, J., Saavedra, R. A., Hood, L. E. & Kent, S. B. H. Proc. natn. Acad. Sci. U.S.A. 84, 6970–6974 (1987).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Nagai, K., Oubridge, C., Jessen, T. H., Li, J. & Evans, P. Nature 348, 515–520 (1990).
Hoffman, D. W., Query, C. C., Golden, B., White, S. W. & Keene, J. D. Proc. natn. Acad. Sci. U.S.A. 88, 2495–2499 (1991).
Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Raabe, T., Bollum, F. & Manley, J. Primary structure and expression of bovine poly(A) polymerase. Nature 353, 229–234 (1991). https://doi.org/10.1038/353229a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/353229a0
This article is cited by
-
A PtdIns4,5P2-regulated nuclear poly(A) polymerase controls expression of select mRNAs
Nature (2008)
-
The effect of the polyadenylation inhibitor cordycepin on human Molt-4 and Daudi leukaemia and lymphoma cell lines
Cancer Chemotherapy and Pharmacology (2008)
-
The role of cordycepin in cancer treatment via induction or inhibition of apoptosis: implication of polyadenylation in a cell type specific manner
Cancer Chemotherapy and Pharmacology (2007)
-
Cell-cycle related regulation of poly(A) polymerase by phosphorylation
Nature (1996)
-
Biochemical and immunological identification and enrichment of poly(A) polymerase from human thymus
Molecular and Cellular Biochemistry (1996)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.