Structure of the Vδ domain of a human γδ T-cell antigen receptor

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Abstract

Antigen recognition by T lymphocytes is mediated by cell-surface glycoproteins known as T-cell antigen receptors (TCRs). These are composed of α and β, or γ and δ, polypeptide chains with variable (V) and constant (C) regions. In contrast to αβ TCRs, which recognize antigen only as peptide fragments bound to molecules of the major histocompatibility complex (MHC), γδ TCRs appear to recognize proteins directly, without antigen processing, and to recognize MHC molecules independently of the bound peptide1,2,3,4. Moreover, small phosphate-containing non-peptide compounds have also been identified as ligands for certainγδ T cells5,6. These studies indicate that antigen recognition by γδ TCRs may be fundamentally different from that by αβ TCRs. The three-dimensional structures of several αβ TCRs and TCR fragments7,8,9,10, and their complexes with peptide–MHC or superantigens9,10,11, have been determined. Here we report the crystal structure of the Vδ domain of a human γδ TCR at 1.9 Å resolution. A comparison with antibody and αβ TCR V domains reveals that the framework structure of Vδ more closely resembles that of VHthan of Vα, Vβ or VL(where H and L refer to heavy and light chains), whereas therelative positions and conformations of its complementarity-determining regions (CDRs) share features of both Vα and VH. These results provide the first direct evidence that γδ TCRs are structurally distinct from αβ TCRs and, together with the observation that the CDR3 length distribution of TCR δ chains is similar to that of immunoglobulin heavy chains12, are consistent with functional studies suggesting that recognition of certain antigens by γδ TCRs may resemble antigen recognition by antibodies1,2,3.

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Figure 1: Structure of the TCR Vδ domain (Vδ3-Dδ2-Jδ1).
Figure 2: Alignment of the ES204 Vδ sequence with immunoglobulin and TCR V domain sequences based on structural information.
Figure 3: Comparison of Vδ with Vα, Vβ, VHand VLstructures.
Figure 4: The antigen-binding loops of ES204 Vδ.

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Acknowledgements

We thank X. Ysern and B. C. Braden for valuable discussions, X. Ji for help in map interpretation, R. J. Poljak for critical reading of the manuscript, and the staff at Brookhaven National Laboratory for assistance with data collection. We also thank I. A. Wilson for providing coordinates of the 2C TCR and D. N. Garboczi and D. C. Wiley for coordinates of the A6 TCR. This work was supported by grants from the NIH (R.A.M. and M.B.B.) and the National Multiple Sclerosis Society (R.A.M.).

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Correspondence to Roy A. Mariuzza.

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