Laminar flows

Subcellular positioning of small molecules

Abstract

Localized perturbation of processes that take place inside the living cell depends on molecular and spatial discrimination on a micrometre scale. Here we report the use of multiple laminar streams in a microfluidic channel to deliver membrane-permeable molecules to selected subcellular microdomains. This technique opens up avenues for non-invasively visualizing, probing and manipulating the cellular metabolic and structural machinery.

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Figure 1: Differential manipulation of regions of a single bovine capillary endothelial cell using multiple laminar flows.

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Correspondence to George M. Whitesides.

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