Gene expression

Total silencing by intron-spliced hairpin RNAs

Abstract

Post-transcriptional gene silencing (PTGS), a sequence-specific RNA degradation mechanism inherent in many life-forms, can be induced in plants by transforming them with either antisense1 or co-suppression2 constructs, but typically this results in only a small proportion of silenced individuals. Here we show that gene constructs encoding intron-spliced RNA with a hairpin structure can induce PTGS with almost 100% efficiency when directed against viruses or endogenous genes. These constructs could prove valuable in reverse genetics, genomics, engineering of metabolic pathways and protection against pathogens.

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Figure 1: Efficiency of induction of post-transcriptional gene silencing (PTGS) by different gene constructs and the predicted structure of RNA transcribed from the transgenes.

References

  1. 1

    Hamilton, A. J., Lycett, G. W. & Grierson, D. Nature 346, 284– 287 (1990).

    ADS  CAS  Article  Google Scholar 

  2. 2

    Jorgensen, R. A. Science 268, 689–691 ( 1995).

    ADS  Article  Google Scholar 

  3. 3

    Waterhouse, P. M., Graham, M. W. & Wang, M.-B Proc. Natl Acad. Sci. USA 95, 13959–13964 (1998).

    ADS  CAS  Article  Google Scholar 

  4. 4

    Waterhouse, P. M., Smith, N. A. & Wang, M.-B Trends Plant Sci. 4, 452– 457 (1999).

    CAS  Article  Google Scholar 

  5. 5

    Wang, M.-B, Upadhyaya, M. N., Brettel, R. I. S. & Waterhouse, P. M. J. Genet. Breed. 51, 325–334 (1997).

    CAS  Google Scholar 

  6. 6

    Okuley, J. et al. Plant Cell 6, 147–158 (1994).

    CAS  Article  Google Scholar 

  7. 7

    Cartea, M. E., Migdal, M., Galle, A. M., Pelletier, G. & Guerche, P. Plant Sci. 136, 181– 194 (1998).

    CAS  Article  Google Scholar 

  8. 8

    Rosche, E. & Westhoff, P. Plant Mol. Biol. 29 , 663–678 (1995).

    CAS  Article  Google Scholar 

  9. 9

    Gleave, A. P. Plant Mol. Biol. 20, 1203–1207 (1992).

    CAS  Article  Google Scholar 

  10. 10

    Stalberg, K., Ellerstrom, M., Josefsson, L. G. & Rask, L. Plant Mol. Biol. 23, 671–683 (1993).

    CAS  Article  Google Scholar 

  11. 11

    Fire, A. et al. Nature 391, 806–811 (1998).

    ADS  CAS  Article  Google Scholar 

  12. 12

    Sharp, P. A. Genes Dev. 13, 139–141 ( 1999).

    CAS  Article  Google Scholar 

Download references

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Correspondence to Peter M. Waterhouse.

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brief communications is intended to provide a forum for short, topical reports of general scientific interest and for technical discussion of recently published material of particular interest to non-specialist readers. Priority will be given to contributions that have fewer than 500 words, 10 references and only one figure. Detailed guidelines are available on Nature's website (http://www.nature.com) or on request from nature@nature.com

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Smith, N., Singh, S., Wang, MB. et al. Total silencing by intron-spliced hairpin RNAs. Nature 407, 319–320 (2000). https://doi.org/10.1038/35030305

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