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Abstract

Mice have been cloned by nuclear transfer into enucleated oocytes1,2,3, and here we describe the reiterative cloning of mice to four and six generations in two independent lines. Successive generations showed no signs of prematureageing, as judged by gross behaviouralparameters, and there was no evidence of shortening of telomeres at the ends of chromosomes, normally an indicator of cellular senescence — in fact, these appeared to increase slightly in length. This increase is surprising, given that the number of mitotic divisions greatly exceeds that of sexually produced animals and that any deleterious effects of cloning might be expected to be amplified in sequentially cloned mice. Our results offer a new approach to the study of organismal ageing.

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Author notes

    • Teruhiko Wakayama

    Present address: The Rockefeller University , 1230 York Avenue, New York, New York 10021, USA

Affiliations

  1. *Department of Anatomy and Reproductive Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA

    • Teruhiko Wakayama
    • , Kellie L. K. Tamashiro
    •  & Ryuzo Yanagimachi
  2. ‡Department of Cell Biology, Institute for Virus Research, Kyoto University, Sakyo-Ku, Kyoto 606-8507, Japan

    • Yoichi Shinkai
    •  & Makoto Tachibana
  3. §Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley , Cailfornia 94720, USA

    • Hiroyuki Niida
  4. Bekesy Laboratory of Neurobiology, University of Hawaii, Honolulu, Hawaii 96822 , USA

    • D. Caroline Blanchard
    •  & Robert J. Blanchard
  5. ¶Department of Veterinary Science, National Institute of Infectious Disease, Tokyo 162-8640, Japan

    • Atsuo Ogura
    •  & Kentaro Tanemura
  6. #The Rockefeller University, 1230 York Avenue, New York, New York 10021 , USA

    • Anthony C. F. Perry
    • , Diana F. Colgan
    •  & Peter Mombaerts

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Corresponding author

Correspondence to Teruhiko Wakayama.

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DOI

https://doi.org/10.1038/35030301

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