IT is the prevailing notion that cochlear outer hair cells function as mechanical effectors as well as sensory receptors1–3. Electrically induced changes in the shape of mammalian outer hair cells4,5, studied in vitro, are commonly assumed to represent an aspect of their effector process that may occur in vivo. The nature of the motile process is obscure, even though none of the established cellular motors can be involved6. Although it is known that the motile response is under voltage control7, it is uncertain whether the stimulus is a drop in the voltage along the long axis of the cell or variation in the transmembrane potential. We have now performed experiments with cells partitioned in differing degrees between two chambers. Applied voltage stimulates the cell membrane segments in opposite polarity to an amount dependent on the partitioning. The findings show, in accordance with previous suggestions6–8, that the driving stimulus is a local transmembrane voltage drop and that the cellular motor consists of many independent elements, distributed along the cell membrane and its associated cortical structures. We further show that the primary action of the motor elements is along the longitudinal dimension of the cell without necessarily involving changes in intracellular hydrostatic pressure. This establishes the outer hair cell motor as unique among mechanisms that control cell shape9.
Subscribe to Journal
Get full journal access for 1 year
only $3.90 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Davis, H. Hearing Res. 9, 79–90 (1983).
Dallos, P. in Contemporary Sensory Neurobiology (eds Correia M. J. & Perachio A. A.) 207–230 (Alan R. Liss, New York, 1985).
Kim, D. O. Hearing Res. 22, 105–114 (1986).
Brownell, W. E. in Mechanisms of Hearing (eds Webster W. R. & Aitkin L. M.) 5–10 (Monash Univ. Press, Clayton, Australia, 1983).
Brownell W. E., Bader, C. R., Bertrand, D. & de Ribaupierre, Y. Science 227, 194–196 (1985).
Holley, M. C. & Ashmore, J. F. Proc R. Soc. B232, 413–429 (1988).
Santos-Sacchi, J. & Dilger, J. P. Hearing Res. 35, 143–150 (1988).
Ashmore, J. F. J. Physiol., Lond. 388, 323–347 (1987).
Lackie, J. M. Cell Movement and Cell Behaviour (Allen & Unwin, London, 1986).
Saito, K. Cell Tiss. Res. 229, 467–481 (1983).
Evans, B. N. Hearing Res. 45, 265–282 (1990).
Flock, Å., Flock, B. & Ulfendahl, M. Arch. Otorhinolaryngol. 243, 83–90 (1986).
Holley, M. C. & Ashmore, J. F. Nature 335, 635–637 (1988).
Bannister, L. H., Dodson, H. C., Astbury, A. F. & Douek, E. E. Prog. Brain. Res. 74, 213–219 (1988).
Smith, C. A. & Dempsey, E. W. Am. J. Anat. 100, 337–367 (1957).
Guinan, J. J., Warr, W. B. & Norris, B. E. J. comp. Neurol. 221, 358–370 (1983).
Spoendlin, H. Acta Otolaryngol. (Stockholm) 67, 239–254 (1969).
Dallos, P. & Harris, D. M. J. Neurophysiol. 41, 365–383 (1978).
Dallos, P. in Auditory Function (eds Edelman G. M., Gall W. E. & Cowan W. M. 153–188 (J. Wiley, New York, 1988).
Brownell, W. E. & Kachar, B. in Peripheral Auditory Mechanisms (eds Allen J. B., Hall J. L., Hubbard A., Neely S. T. & Tubis A.) 369–376 (Springer-Verlag, New York, 1986).
Brownell, W. E. Ear and Hearing 11, 82–92 (1990).
Jen, D. H. & Steele, C. R. J. acoust. Soc. Amer. 82, 1667–1678 (1987).
Baylor, D. A., Lamb, T. D. & Yau, K.-W. J. Physiol., Lond. 288, 589–611 (1979).
Holley, M. C. & Ashmore, J. F. J. Cell Sci 96, 283–291 (1990).
Evans, B. N. thesis, Univ. Texas Health Sciences Center, Houston (1988).
Clark, B. A., Hallworth, R. & Evans, B. N. Pflügers Arch. 415, 490–493 (1990).
About this article
Cite this article
Dallos, P., Evans, B. & Hallworth, R. Nature of the motor element in electrokinetic shape changes of cochlear outer hair cells. Nature 350, 155–157 (1991) doi:10.1038/350155a0
Neural Plasticity (2018)
Journal of Neuroscience Methods (2018)
Synchronized Progression of Prestin Expression and Auditory Brainstem Response during Postnatal Development in Rats
Neural Plasticity (2016)
Proceedings of the National Academy of Sciences (2013)
PLoS ONE (2012)