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The crystal structure of the photoprotein aequorin at 2.3 Å resolution

Abstract

Aequorin is a calcium-sensitive photoprotein originally obtained from the jellyfish Aequorea aequorea1. Because it has a high sensitivity to calcium ions and is biologically harmless, aequorin is widely used as a probe to monitor intracellular levels of free calcium. The aequorin molecule contains four helix–loop–helix ‘EF-hand’ domains, of which three can bind calcium2. The molecule also contains coelenterazine as its chromophoric ligand3. When calcium is added, the protein complex decomposes into apoaequorin, coelenteramide and CO2, accompanied by the emission of light4. Apoaequorin can be regenerated into active aequorin in the absence of calcium by incubation with coelenterazine, oxygen and a thiol agent5. Cloning and expression of the complementary DNA for aequorin were first reported in 1985 (refs 2, 6), and growth of crystals of the recombinant protein has been described7; however, techniques have only recently been developed to prepare recombinant aequorin of the highest purity8, permitting a full crystallographic study. Here we report the structure of recombinant aequorin determined by X-ray crystallography. Aequorin is found to be a globular molecule containing a hydrophobic core cavity that accommodates the ligand coelenterazine-2-hydroperoxide. The structure shows protein components stabilizing the peroxide and suggests a mechanism by which calcium activation may occur.

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Figure 1: Ribbon representation showing the secondary structure elements in the protein.
Figure 2: Model of peroxidized coelenterazine with Tyr 184 and His 169.
Figure 3: Stereo view of peroxidized coelenterazine (at centre) with some of the surrounding residues.
Figure 4: Representation of peroxidized coelenterazine showing all distances to protein atoms within 3.6 Å and some other local interactions.

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Acknowledgements

We thank B. Kaminer for initiating this collaborative effort; B. Seaton for involvement in early crystallization studies; H. Nakamura for structural information on imidazopyrazinone; and the Boston University Mass Spectrometry Facility. This work was supported in part by NSF grants to O.S. and to J.F.H.

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Correspondence to James F. Head.

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Head, J., Inouye, S., Teranishi, K. et al. The crystal structure of the photoprotein aequorin at 2.3 Å resolution . Nature 405, 372–376 (2000). https://doi.org/10.1038/35012659

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