Letter | Published:

Segregation of a missense mutation in the amyloid precursor protein gene with familial Alzheimer's disease

Abstract

A LOCUS segregating with familial Alzheimer's disease (AD) has been mapped to chromosome 21 (ref. 1), close to the amyloid precursor protein (APP) gene2–5. Recombinants between the APP gene and the AD locus have been reported6–8 which seemed to exclude it as the site of the mutation causing familial AD. But recent genetic analysis of a large number of AD families has demonstrated that the disease is heterogeneous9. Families with late-onset AD do not show linkage to chromosome 21 markers9,10. Some families with early-onset AD show linkage to chromosome 21 markers, but some do not8,9,11. This has led to the suggestion that there is non-allelic genetic heterogeneity even within early onset familial AD8,9. To avoid the problems that heterogeneity poses for genetic analysis, we have examined the cosegregation of AD and markers along the long arm of chromosome 21 in a single family with AD confirmed by autopsy. Here we demonstrate that in this kindred, which shows linkage to chromosome 21 markers, there is a point mutation in the APP gene. This mutation causes an amino-acid substitution (Val→Ile) close to the carboxy terminus of the β-amyloid peptide. Screening other cases of familial AD revealed a second unrelated family in which this variant occurs. This suggests that some cases of AD could be caused by mutations in the APP gene.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    St George-Hyslop, P. et al. Science 235, 885–890 (1987).

  2. 2

    Kang, J. et al. Nature 325, 733–736 (1987).

  3. 3

    Goldgaber, D., Lerman, M. I., McBride, O. W., Sappiotti, U. & Gajdusek, D. C. Science 235, 877–880 (1987).

  4. 4

    Robakis, N. K., Ramakrishna, N., Wolfe, G. & Wisniewski, H. M. Proc. natn. Acad. Sci. U.S.A. 84, 4190–4194 (1987).

  5. 5

    Tanzi, R. et al. Science 235, 880–884 (1987).

  6. 6

    Van Broeckhoven, C. et al. Nature 329, 153–155 (1987).

  7. 7

    Tanzi, R. et al. Nature 329, 156–157 (1987).

  8. 8

    Schellenberg, G. et al. Science 241, 1507–1510 (1988).

  9. 9

    St George-Hyslop, P. et al. Nature 347, 194–197 (1990).

  10. 10

    Pericak-Vance, M. et al. Expl Neurol. 102, 271–279 (1988).

  11. 11

    Goate, A. et al. Lancet 1, 352–355 (1989).

  12. 12

    Tanzi, R. et al. Genomics 3, 129–136 (1988).

  13. 13

    Warren, A. et al. Genomics 4, 579–591 (1989).

  14. 14

    Lewis, G. et al. Genomics 8, 400–402 (1990).

  15. 15

    Guo, Z., Sharma, V., Patterson, D. & Litt, M. Nucleic Acids Res. 18, 4967 (1990).

  16. 16

    Kidd, K. et al. Cytogenet. Cell Genet. 51, 622–947 (1989).

  17. 17

    Lathrop, G. M., Lalouel, J. M., Julier, C. & Ott, J. Proc. natn. Acad. Sci. U.S.A. 81, 3443–3446 (1984).

  18. 18

    Gyllensten, U. in PCR Technology (ed. Erlich, H. A.) 45–60 (Stockton, London, 1989).

  19. 19

    Yoshikai, S., Sasaki, H., Doh-ura, K., Furnya, H. & Sakaki, Y. Gene 87, 257–263 (1990).

  20. 20

    Van Broeckhoven, C. et al. Science 248, 1120–1123 (1990).

  21. 21

    Levy, E. et al. Science 248, 1124–1126 (1990).

  22. 22

    Barker, D., Schafer, M. & White, R. Cell 36, 131–138 (1984).

  23. 23

    Argos, P., Rao, J. K. M. & Hargrave, P. A. Eur. J. Biochem. 128, 565–575 (1982).

  24. 24

    Garnier, J., Osguthorpe, D. J. & Robson, B. J. molec. Biol. 120, 97–120 (1978).

  25. 25

    Gaboriaud, C., Bissery, V., Benchetrit, T. & Mornon, J. P. FEBS Lett. 224, 149–155 (1987).

  26. 26

    Kyte, J. & Doolittle R. F. J. molec. Biol. 157, 105–132 (1982).

  27. 27

    Levin, J., M., Robson, B. & Garnier, J. FEBS Lett. 205, 303–308 (1986).

  28. 28

    Lim, V. J. molec. Biol. 88, 857–872 (1974).

  29. 29

    Gibrat, J., Garnier, J. & Robson, B. J. molec. Biol. 198, 425–443 (1987).

  30. 30

    Glenner, G. G. & Wong, C. W. Biochem. biophys. Res. Commun. 120, 1131–1135 (1984).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.