Abstract
THE bcl-2 gene is consistently associated with t(14; 18) chromosomal translocations1–6 observed in a large fraction of human B-cell lymphomas7–8. The t(14; 18) translocation results in deregulated expression of the bcl-2 gene5 and synthesis of inappropriately high levels of the Bcl-2 protein9,10. Gene transfer studies suggest a role for Bcl-2 in cell survival11,12, growth enhancement13,14 and oncogenic transformation11,15. To test the suggestion that GTP-binding by Bcl-2 may mediate its biological effects16 we characterized the GTP-binding proteins in lymphoid cells expressing Bcl-2. Expression of several small GTP-binding proteins was found to be ubiquitous and did not vary with levels of Bcl-2. By using immunological, electrophoretic and cell-fractionation techniques, we separated Bcl-2 from G proteins of small relative molecular mass (Mr) and showed that it is incapable of binding GTP. Our results show that small Mr G proteins are widely expressed in lymphoid cells and that Bcl-2 is not a novel member of this GTP-binding protein family.
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References
Tsujimoto, Y., Finger, L. R., Yunis, J., Nowell, P. C. & Croce, C. Science 226, 1097–1099 (1984).
Bakhshi, A. et al. Cell 41, 899–906 (1985).
Cleary, M. L. & Sklar, J. Proc. natn. Acad. Sci. U.S.A. 82, 7439–7443 (1985).
Cleary, M. L., Galili, N. & Sklar, J. J. exp. Med. 164, 315–320 (1986).
Cleary, M. L., Smith, S. D. & Sklar, J. Cell 47, 19–28 (1986).
Weiss, L. M., Warnke, R. A., Sklar, J. & Cleary, M. L. New Engl. J. Med. 317, 1185–1189 (1987).
Fukuhara, S., Rowley, J. D., Variakojis, D. & Golomb, H. M. Cancer Res. 39, 3119–3128 (1979).
Yunis, J. J. et al. New Engl. J. Med. 307, 1231–1236 (1982).
Ngan, B., Chen-Levy, Z., Weiss, L. M., Warnke, R. M. & Cleary, M. L. New Engl. J. Med. 318, 1638–1644 (1988).
Chen-Levy, Z., Nourse, J. & Cleary, M. L. Molec. cell. Biol. 9, 701–710 (1989).
Vaux, D. L., Cory, S. & Adams, J. M. Nature 335, 440–442 (1988).
McDonnell, T. J. et. al. Cell 57, 79–88 (1989).
Reed, J. C., Haldar, S., Cuddy, M. P., Croce, C. & Makover, D. Oncogene 4, 1123–1127 (1989).
Tsujimoto, Y. Proc. natn. Acad. Sci. U.S.A. 86, 1958–1962 (1989).
Reed, J. C., Cuddy, M., Slabiak, T., Croce, C. M. & Nowell, P. C. Nature 336, 259–261 (1988).
Haldar, S., Beatty, C., Tsujimoto, Y. & Croce, C. M. Nature 342, 195–198 (1989).
Lapetina, E. G. & Reep, B. R. Proc. natn. Acad. Sci. U.S.A. 84, 2261–2265 (1987).
Bokock, G. M. & Parkos, C. A. FEBS Lett. 227, 66–70 (1988).
Stryer, L. & Bourne, H. R. A. Rev. Cell Biol. 2, 391–419 (1986).
Carney, W. P., DeLellis, R. A., Wolfe, H., Cooper, G. & Rabin, H. Proc. Am. Ass. Cancer Res. 28, 297 (1987).
Lacal, J. C., Anderson, P. S. & Aaronson, S. A. EMBO J. 5, 679–687 (1986).
Santos, E. & Nebreda, A. R. Fedn Proc. Fedn. Am. Socs exp. Biol. 3, 2151–2163 (1989).
Nigg, E. A., Walter, G. & Singer, S. J. Proc. natn. Acad. Sci. U.S.A. 79, 5939–5943 (1982).
Chardin, P. & Tavitian, A. EMBO J. 5, 2203–2208 (1986).
Takai, Y. et al. in Progress in Endocrinology Vol. 2 (eds Imura, H., Shizume, K. & Yoshida, S.) 995–1000 (Elsevier, Amsterdam, 1988).
Ohmori, T. et al. Biochem. biophys. Res. Commun. 157, 670–676 (1988).
Pizon, V., Chardin, P., Lerosey, I., Olofsson, B. & Tavitian, A. Oncogene 3, 201–204 (1988).
Kitayama, H., Sugimoto, Y., Matsuzaki, T., Ikawa, Y. & Noda, M. Cell 56, 77–84 (1989).
Nagata, K. et al. J. Biol. Chem. 264, 17000–17005 (1989).
Chen-Levy, Z. & Cleary, M. L. J. biol. Chem. 265, 4929–4933 (1990).
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Monica, K., Chen-Levy, Z. & Cleary, M. Small G proteins are expressed ubiquitously in lymphoid cells and do not correspond to Bcl-2. Nature 346, 189–191 (1990). https://doi.org/10.1038/346189a0
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DOI: https://doi.org/10.1038/346189a0
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