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Probing the calcium-induced conformational transition of troponin C with site-directed mutants

Nature volume 345, pages 182–184 (1990)Cite this article

Abstract

THE contraction of skeletal muscle is regulated by calcium binding to troponin C (TnC ; Fig. 1)1. TnC consists of two spatially independent domains, each of which contains two metal ion binding sites2–5. Calcium binding to the regulatory sites of the N-terminal domain6,7 triggers muscle contraction by a series of conformational changes. Site-directed mutagenesis offers a means of elucidating the links in this signal path between TnC and actin–myosin crossbridges. Such mapping is possible if the mutants shift the equilibrium between 'on' and 'off' states of the regulatory complex while maintaining the coupling between calcium binding and tension development. Candidate amino-acid residues for yielding this information would be in positions remote from the calcium-binding sites and from the site of development of tension. Analysis of the crystal structure of TnC2,8,9 and of the model of the calcium-activated molecule10 has enabled us to identify two such residues: Glu 57 and Glu 88. In separate experiments we have replaced each of these residues by lysines. The resulting reduction in calcium affinity indicates that these residues have a long-range effect on calcium binding. This result may reflect the formation of a salt bridge between positions 57 and 88 that is not present in the native molecule. Moreover, the level of tension recovery when the mutants are incorporated into muscle suggests that the interaction between TnC and other muscle components has also been altered. Thus, these residues may participate in the contraction signal transmission.

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Author notes

  1. F. C. Reinach: To whom correspondence should be addressed.

Authors and Affiliations

  1. Departamento de Bioquca, Instituto de Quca, Universidade de Sao Paulo, CP 20 780, CEP 01498, Sao Paulo, Brazil

    K. Fujimori & F. C. Reinach

  2. Departamento de Bioquca, Instituto de Ciencias Biomedicas,Universidade Federal do Rio de Janeiro, CEP 21949, Rio de Janeiro, Brazil

    M. Sorenson

  3. Center for Advanced Research in Biotechnology, The Maryland Biotechnology Institute, University of Maryland, Rockville, Maryland, 20850, USA

    O. Herzberg & J. Moult

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  1. K. Fujimori
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  4. J. Moult
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  5. F. C. Reinach
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Fujimori, K., Sorenson, M., Herzberg, O. et al. Probing the calcium-induced conformational transition of troponin C with site-directed mutants. Nature 345, 182–184 (1990). https://doi.org/10.1038/345182a0

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