Abstract
RECENT experiments have implicated intracellular events in the formation of the MHC class II-peptide complexes recognized by CD4-positive T cells1–6. These data raise the possibility that the intracellular association of class II with the non-polymorphic glycoprotein, invariant chain (Ii)7,8, may regulate the interaction between processed antigen and MHC class II molecules. To address this possibility, we have generated a series of transfected fibroblast cell lines that express class II with and without Ii. Although the presence of Ii does not seem to affect the ability of the cells to process and present intact antigen, Ii-negative cells express an altered form of class II at the cell surface. This modified conformation of class II in Ii-negative cells is detectable by an increase in the ability to present antigenic peptides to T cells and a decrease in the binding of several class II-specific monoclonal antibodies.
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References
Unanue, E. A. Rev. Immun. 2, 395–428 (1984).
Buus, S., Sette, A., Colon, S., Jenis, D. & Grey, H. Cell 47, 1071–1077 (1986).
Morrison, L., Lukacher, A., Brachiale, V., Fan, D. & Brachiale, T. J. exp. Med. 163, 903–921 (1986).
Cresswell, P. Proc. natn. Acad. Sci. U.S.A. 82, 8188–8192 (1985).
Harding, C. & Unanue, E. J. Immun. 142, 12–19 (1989).
Jensen, P. J. immun. 141, 2545–2550 (1988).
Kvist, S., Wiman, K., Claesson, L., Peterson, P. & Dobberstein, B. Cell 29, 61–69 (1982).
Holt, G., Swiedler, S., Freed, J. & Hart, G. J. Immun. 135, 399–407 (1985).
Miller, J. & Germain, R. J. exp. Med 164, 1478–1489 (1986).
Sekaly, R., Tonnelle, C., Strubin, M., Mach, B. & Long, E. J. exp. Med. 164, 1490–1504 (1986).
Simonis, S., Miller, J. & Cullen, S. J. Immun. 143, 3619–3625 (1989).
Shimonkevitz, R., Kappler, J., Marrack, P. & Grey, H. J. exp. Med. 158, 303–316 (1983).
Townsend, A. et al. Nature 340, 443–448 (1989).
Mellins, E. et al. Nature 343, 71–74 (1990).
Germain, R. & Malissen, B. A. Rev. Immun. 4, 281–315 (1986).
Stockinger, B. et al. Cell 56, 683–689 (1989).
Miller, J., Hatch, J., Simonis, S. & Cullen, S. Proc. natn. Acad. Sci. U.S.A. 85, 1359–1363 (1988).
Glimcher, L., Sohroer, J., Chan, C. & Shevach, E. J. Immun. 131, 2868–2874 (1983).
Kappler, J., Scidmore, B., White, J. & Marrack, P. J. exp. Med. 153, 1198–1214 (1981).
Ozato, K., Mayer, N. & Sachs, D. Transplantation 34, 113–120 (1982).
Bhattacharya, A., Dorf, M. & Springer, T. J. Immun. 127, 2488–2495 (1981).
Ozato, K. & Sachs, D. J. Immun. 126, 317–321 (1981).
Koch, N., Hammerling, G., Tada, N., Kimura, S. & Hammerling, U. Eur. J. Immun. 12, 909–914 (1982).
Pierres, M., Devaux, C., Dosseto, M. & Marchetto, S. Immunogenetics 14, 481–495 (1981).
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Peterson, M., Miller, J. Invariant chain influences the immunological recognition of MHC class II molecules. Nature 345, 172–174 (1990). https://doi.org/10.1038/345172a0
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DOI: https://doi.org/10.1038/345172a0
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