The polymerase chain reaction is used for site-specific mutagenesis and for DNA recombination without any enzymatic reaction in vitro, apart from DNA amplification.
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Jones, D., Sakamoto, K., Vorce, R. et al. DMA mutagenesis and recombination. Nature 344, 793–794 (1990). https://doi.org/10.1038/344793a0
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DOI: https://doi.org/10.1038/344793a0
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