Abstract
IN higher eukaryotes, steroid receptors are general modulators of gene activity which bind to DNA hormone response elements (HREs) in the vicinity of regulated promoters1. Analysis of mutant and chimaeric receptor proteins in gene transfer experiments has identified the domains responsible for hormone binding, interaction with the HREs and transactivation2, but the transactivating func-tion of hormone receptors has proved difficult to reproduce in cell-free assays. Here we describe a crude in vitro system in which transcription from the mouse mammary tumour virus (MMTV) promoter is increased up to 10-fold by native progesterone receptor from rabbit uterus. The stimulatory effect must depend on receptor binding to the MMTV-HRE as it is abolished by deletion or mutation of the HRE and by HRE-oligonucleotide competition. The nuclear factor-I binding site immediately downstream of the HRE, however, does not appear to be essential to progesterone receptor-mediated in vitro stimulation of MMTV transcription. The transactivation activity of the progesterone receptor depends on binding of a functional ligand and so this assay should be useful for dissection of the mechanism of transcription activation by steroid hormones.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Beato, M. Cell 56, 335–344 (1989).
Evans, R. M. Science 240, 889–895 (1988).
Dignam, J. D., Lebovitz, R. M. & Roeder, R. G. Nucleic Acids Res. 11, 1475–1489 (1983).
Ringold, G. M. Biochim. biophys. Acta 560, 487–508 (1979).
von der Ahe, D. et al. Nature 313, 706–709 (1985).
Chalepakis, G. et al. Cell 53, 371–382 (1988).
Loosfelt, H., Logeat, F., Hai, M. T. V. & Milgrom, E. J. biol. Chem. 259, 14196–14202 (1984).
Schauer, M., Chalepakis, G., Willmann, T. & Beato, M. Proc. natn. Acad. Sci. U.S.A. 86, 1123–1127 (1989).
Cato, A. B. C., Skroch, P., Weinmann, J., Butkeraitis, P. & Ponta, H. EMBO J. 7, 1403–1410 (1988).
Nowock, J., Borgmeyer, U., Püschel, A. W., Rupp, R. A. W. & Sippel, A. E. Nucleic Acids Res. 13, 2045–2061 (1985).
Buetti, E. & Külhnel, B. J. molec. Biol. 190, 379–389 (1986).
Miksicek, R., Borgmeyer, U. & Nowock, J. EMB0 J. 6, 1355–1360 (1987).
Meisterernst, M., Gander, I., Rogge, L. & Winnacker, E-L. Nucleic Acids Res. 16, 4419–4435 (1988).
Guiochon-Mantel, A. et al. Nature 336, 695–698 (1988).
Pin̄a, B., Brüggemeier, U. & Beato, M. Cell (in the press).
Zinn, K., DiMaio, D. & Maniatis, T. Cell 34, 865–879 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kalff, M., Gross, B. & Beato, M. Progesterone receptor stimulates transcription of mouse mammary tumour virus in a cell-free system. Nature 344, 360–362 (1990). https://doi.org/10.1038/344360a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/344360a0
This article is cited by
-
Chromatin structure of the MMTV promoter and its changes during hormonal induction
Cellular and Molecular Neurobiology (1996)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
