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Identification of classical minor histocompatibility antigen as cell-derived peptide


HISTOCOMPATIBILITYantigens expressed on tissue grafted between individuals are recognized by host T cells, which reject the graft1,2. The major histocompatibility complex (MHC) antigens have been identified on the molecular level, whereas the molecules representing the remaining ones, the minor histocompatibility antigens, are unknown, apart from some exceptions3–5. The cytotoxic T lymphocyte (CTL) response against minor histocompatibility antigens shares many aspects with that against virus-infected cells6,7. Virus-specific CTL recognize peptides derived from viral proteins produced in the infected cell. These peptides are presented by MHC class I molecules, as indicated by functional and crystallographic data8,9. By analogy, minor histocompatibility antigens have been postulated to be peptides derived from normal cellular proteins presented by MHC class I molecules10–12. Here we report that peptides derived from normal cellular proteins can indeed be recognized by CTL raised in the classical minor histo-incompatible mouse strain combination, C57BL/6 against BALB.B. Thus, we have proven the above postulate, and isolated one of the minor histocompatibility molecules elusive for several decades.

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  1. Snell, G. D. Genetics 49, 87–108 (1948).

    Article  CAS  Google Scholar 

  2. Klein, J. Natural History of the Major Histocompatibility Complex (Wiley, New York, 1986).

    Google Scholar 

  3. Counce, S., Smith, P., Barth, R. & Snell, G. D. Ann. Surgery 144, 198–204 (1956).

    Article  Google Scholar 

  4. Loveland, B. & Simpson, E. Immun. Today 7, 223–229 (1986).

    Article  CAS  Google Scholar 

  5. Wettstein, P. J. in Human Immunogenetics (ed. Litwin, S. D.) 339–357 (Dekker, New York, 1989).

    Google Scholar 

  6. Zinkernagel, R. M. & Doherty, P. C. Nature 248, 701–702 (1974).

    Article  ADS  CAS  Google Scholar 

  7. Bevan, M. J. Nature 256, 419–421 (1975).

    Article  ADS  CAS  Google Scholar 

  8. Townsend, A. R. M. et al. Cell 44, 959–968 (1986).

    Article  CAS  Google Scholar 

  9. Bjorkman, P. J. et al. Nature 329, 512–518 (1987).

    Article  ADS  CAS  Google Scholar 

  10. Claverie, J.-M. & Kourilsky, P. Ann. Inst. Pasteur, Paris 137D, 425–442 (1986).

    CAS  Google Scholar 

  11. Germain, R. Nature 322, 687–689 (1986).

    Article  ADS  CAS  Google Scholar 

  12. Bevan, M. J. Nature 325, 192–194 (1987).

    Article  ADS  CAS  Google Scholar 

  13. Rammensee, H. G., Robinson, P. J., Crisanti, A. & Bevan, M. J., Nature 319, 502–504 (1986).

    Article  ADS  CAS  Google Scholar 

  14. De Plaen, E. et al. Proc. natn. Acad. Sci. U.S.A. 85, 2274–2278 (1988).

    Article  ADS  CAS  Google Scholar 

  15. Carbone, F. R. & Bevan, M. J. J. exp. Med. 169, 603–612 (1989).

    Article  CAS  Google Scholar 

  16. Rammensee, H.-G., Schild, H. & Theopold, U. Immunogenetics 30, 296–302 (1989).

    Article  CAS  Google Scholar 

  17. Klein, J., Figueroa, F. & David, C. S. Immunogenetics 17, 553–596 (1983).

    Article  CAS  Google Scholar 

  18. Graff, R. J. in Origins of Inbred Mice (ed. Morse, H. C. III) 667–687 (Academic, New York, 1979).

    Google Scholar 

  19. Krauspe, R. & Scheer, A. Analyt. Biochem. 153, 242–250 (1986).

    Article  CAS  Google Scholar 

  20. Rüsch. E, Kuon, W. & Hämmerling, G. J. Transplantn. Proc. 15, 2093–2096 (1983).

    Google Scholar 

  21. Lemke, H. Hämmerling, G. J. & Hämmerling, U. Immunol. Rev. 47, 175–206 (1979).

    Article  CAS  Google Scholar 

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Wallny, HJ., Rammensee, HG. Identification of classical minor histocompatibility antigen as cell-derived peptide. Nature 343, 275–278 (1990).

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