Abstract
GLIA-derived nexin1 (GDN), also known as protease nexin I2,3, is a serine protease inhibitor of deduced relative molecular mass 41,700, identified in conditioned media of glioma cells by its neurite-promoting activity4. GDN can promote neurite outgrowth in vitro from neuroblastoma cells5,6, sympathetic neurons7 and hippocampal neurons (L. Farmer et al., manuscript in preparation). In vivo, GDN is constitutively expressed in all parts of the olfactory system8, where axonal regeneration and neurogenesis occur continuously throughout life. This observation indicates that GDN could be important for axonal regeneration in vivo. To investigate this possibility, we have taken advantage of the fact that damage to nerves in the peripheral nervous system leads to their regeneration, whereas in the central nervous system no such regeneration can occur. Here we report that after lesion of the rat sciatic nerve there is a large transient increase in the amount of GDN messenger RNA and of released GDN. The cells showing GDN immunoreactivity are mainly localized distal to the lesion site. These results further support the suggestion that GDN is important for axonal regeneration in vivo, and indicate that protease inhibitors could have a role in Wallerian degeneration and peripheral nerve regeneration.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Sommer, J. et al. Biochemistry 26, 6407–6410 (1987).
Baker, J. B., Low, D. A., Simmer, R. L. & Cunningham, D. D. Cell 21, 37–45 (1980).
McGrogan, M. et al. Biotechnology 6, 172–177 (1988).
Monard, D., Solomon, F., Rentsch, M. & Gysin, R. Proc. natn. Acad. Sci. U.S.A. 70, 1894–1897 (1973).
Monard, D., Niday, E., Limat, A. & Solomon, F. Prog. Brain Res. 58, 359–364 (1983).
Gurwitz, D. & Cunningham, D. D. Proc. natn. Acad. Sci. U.S.A. 85, 3440–3444 (1988).
Zurn, A. D., Nick H. & Monard, D. Devl. Neurosci. 10, 17–24 (1988).
Reinhard, E., Meier, R., Halfter, W., Rovelli, G. & Monard, D. Neuron 1, 387–394 (1988).
Perry, V. H., Brown, M. C. & Gordon, S., J. exp. Med. 165, 1218–1223 (1987).
Dijkstra, C. D., Doepp, E. A., Joling, P. & Kraal, G. Immunology 54, 589–599 (1985).
Campell, D. G., Gagnon, J., Reid, K. B. M. & Williams, A. F. Biochem. J. 195, 15–30 (1981).
Gery, J., Gershon, R. K. & Waksman, B. J. exp. Med. 136, 128–133 (1972).
Lindholm, D., Heumann, R., Meyer, M. & Thoenen H. Nature 330, 658–659 (1987).
Heumann, R. et al. Proc. natn. Acad. Sci. U.S.A. 84, 8735–8739 (1987).
Martini, R. & Schachner, M. J. Cell Biol. 106, 1735–1746 (1988).
Bergman, B. L., Scott, R. W., Bajpai, A., Watts, S. & Baker, J. B. Proc. natn. Acad. Sci. U.S.A. 83, 996–1000 (1986).
Bignami, A., Cella, G. & Chi, N. H. Acta neuropath. 58, 224–228 (1982).
Farrell, D. H., Wagner, S. L., Yuan, R. H. & Cunningham, D. D. J. Cell Physiol. 134, 179–188 (1988).
Laiho, M., Saksela, O., Andreasen, P. A. & Keski-Oja, J. J. Cell Biol. 103, 2403–2410 (1986).
Gronke, R. S., Knauer, D. J., Veeraraghavan, S. & Baker, J. B. Blood 73, 472–478 (1989).
Wagner, S. L., Lau, A. L. & Cunningham, D. D. J. biol. Chem. 264, 611–615 (1989).
Schwab, M. E. & Thoenen, H. J. Neurosci. 5, 2415–2423 (1985).
Glisin, V., Crkvenjakov, R. & Byus, C. Biochemistry 13, 2633–2637 (1974).
Gloor, S., Odink, K., Guenther, J., Nick, H.-P. & Monard, D. Cell 47, 687–693 (1986).
Hsu, S. M., Raine, L. & Fanger, H. J. Histochem. Cytochem. 29, 577–580 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Meier, R., Spreyer, P., Ortmann, R. et al. Induction of glia-derived nexin after lesion of a peripheral nerve. Nature 342, 548–550 (1989). https://doi.org/10.1038/342548a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/342548a0
This article is cited by
-
Protease nexin 1 in the murine kidney: Glomerular localization and up-regulation in glomerulopathies
Kidney International (1996)
-
Differential expression of triiodothyronine receptors in schwannoma and neurofibroma: role of Schwann cell-axon interaction
Acta Neuropathologica (1995)
-
The neurite growth promoting protease nexin 1 in glial cells of the olfactory bulb of the gerbil: An ultrastructural study
Cell and Tissue Research (1994)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
