Abstract
Direct chemical 'footprinting' shows that translocation of transfer RNA occurs in two discrete steps. During the first step, which occurs spontaneously after the formation of the peptide bond, the acceptor end of tRNA moves relative to the large ribosomal subunit resulting in 'hybrid states' of binding. During the second step, which is promoted by elongation factor EF-G, the anticodon end of tRNA, along with the messenger RNA, moves relative to the small ribosomal subunit.
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References
Hardesty, B. & Kramer, G. (eds) Structure, Function and Genetics of Ribosomes (Springer, New York, 1986).
Watson, J. D. Bull. Soc. chim. Biol. 46, 1399–1425 (1964).
Rheinberger, H., Sternbach, H. & Nierhaus, K. H. Proc. natn. Acad. Sci. U.S.A. 78, 5310–5314 (1981).
Grajevskaja, R. A., Ivanov, Y. V. & Saminsky, E. M. Eur. J. Biochem. 128, 47–52 (1982).
Kirillov, S. V., Makarov, E. M. & Semenkov, Yu. P. FEBS Lett. 157, 91–94 (1983).
Lill, R., Robertson, J. M. & Wintermeyer, W. Biochemistry 23, 6710–6717 (1984).
Wettstein, F. O. & Noll, H. J. Molec. Biol. 11, 35–53 (1965).
Hardesty, B., Culp, W. & McKeehan, W. Cold Spring Harb. Symp. quant. Biol. 34, 331–345 (Cold Spring Harbor, New York, 1969).
Robin, D. & Hardesty, B. Biochemistry 22, 5675–5679 (1983).
Lake, J. A. Proc. natn. Acad. Sci. U.S.A. 74, 1903–1907 (1977).
Skogerson, L. & Moldave, K. Archs Biochem. Biophys. 125, 497–505 (1968).
Noller, H. F. A. Rev. Biochem. 53, 119–162 (1984).
Moazed, D., Stern, S. & Noller, H. F. J. Molec. Biol. 187, 399–416 (1986).
Stern, S., Moazed, D. & Noller, H. F. Meth. Enzym. 164, 481–489 (1988).
Moazed, D. & Noller, H. F. Cell 47, 985–994 (1986).
Moazed, D. & Noller, H. F. J. molec. Biol. (in the press).
Moazed, D. & Noller, H. F. Cell 57, 585–597 (1989).
Traut, R. R. & Monro, R. E. J. Molec. Biol. 10, 63–72 (1964).
Lill, R., Robertson, J. M. & Wintermeyer, W. Biochemistry 25, 3245–3255 (1986).
Gupta, S. L., Waterson, J., Sopori, M. L., Weissman, S. M. & Lengyel, P. Biochemistry 10, 4410–4421 (1971).
Thach, S. S. & Thach, R. E. Proc. natn. Acad. Sci. U.S.A. 68, 1791–1795 (1971).
Pestka, S. J. biol. Chem. 243, 2810–2820 (1968).
Gavrilova, L. P. & Spirin, A. S. FEBS Letts. 17, 324–326 (1971).
de Groot, N., Panet, A. & Lapidot, Y. Eur. J. Biochem. 23, 523–527 (1971).
Watanabe, S. J. Molec. Biol. 67, 443–457 (1972).
Watanabe, S. J. molec. Biol. 114, 135–148 (1966).
Lill, R. et al. J. molec. Biol. 203, 699–705 (1988).
Moazed, D., Robertson, J. M. & Noller, H. F. Nature 334, 362–364 (1988).
Hopfield, J. J. Proc. natn. Acad. Sci. U.S.A. 71, 4135–4139 (1974).
Ninio, J. Biochimie 57, 587–595 (1975).
Hardesty, B., Odom, O. W. & Deng, H.-Y. in Structure, Function and Genetics of Ribosomes (eds Hardesty, B. & Kramer, G.) 495–508 (Springer, New York, 1986).
Odom, O. M. & Hardesty, B. Biochimie 69, 925–938 (1987).
Gassen, H. G. Prog. Nucleic Acid Res. molec. Biol. 24, 57–86 (1980).
Moras, D. et al. J. Biomolec. Str. Dynam. 3, 479–493 (1985).
Lake J. A. in Ribosomes: Structure, Function and Genetics (eds Chamblis, G. et al.) 207–236 (University Park, Baltimore, 1980).
Bretscher, M. S. Nature 218, 675–677 (1968).
Spirin, A. S. Cold Spring Harb. Symp. quant. Biol. 34, 197–207 (Cold Spring Harbor, New York, 1969).
Bretscher, M. S. Nature 218, 675–677 (1968).
Moazed, D. & Noller, H. F. Nature 327, 389–394 (1987).
Nirenberg, M. & Leder, P. Science 145, 1399–1407 (1964).
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Moazed, D., Noller, H. Intermediate states in the movement of transfer RNA in the ribosome. Nature 342, 142–148 (1989). https://doi.org/10.1038/342142a0
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DOI: https://doi.org/10.1038/342142a0
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