Abstract
The three-dimensional crystal structure of met repressor, in the presence or absence of bound corepressor (S-adenosylmethionine), shows a dimer of intertwined monomers, which do not have the helix-turn-helix motif characteristic of other bacterial repressor and activator structures. We propose that the interaction of met repressor with DNA occurs through either a pair of symmetry-related α-helices or a pair of β-strands, and suggest a model for binding of several dimers to met operator regions.
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References
Cohen, G. N. & Jacob, F. C. R. Acad. Sci., Paris 248, 3490–3492 (1959).
Saint-Girons, I., Parsot, C., Zakin, M. M., Bârzu, O. & Cohen, G. N. CRC Critical Reviews in Biochemistry 23, (CRC, Cleveland, Ohio, 1988).
Pabo, C. O. & Lewis, M. Nature 298, 443–447 (1982).
Anderson, W. F., Ohlendorf, D. H., Takeda, Y. & Matthews, B. W. Nature 290, 754–758 (1981).
Mondragon, A., Subbiah, S., Almo, S. C., Drottar, M. & Harrison, S. C. J. molec. Biol. 205, 189–200 (1989).
Mondragon, A., Wolberger, C. & Harrison, S. C. J. molec. Biol. 205, 179–188 (1989).
McKay, D. B., Weber, I. T. & Steitz, T. A. J. biol. Chem. 257, 9518–9524 (1982).
Schevitz, R. W., Otwinowski, Z., Joachimiak A., Lawson, C. L. & Sigler, P. B. Nature 317, 782–786 (1985).
Otwinowski, Z. et al. Nature 335, 321–329 (1988).
Aggarwal, A. K., Rodgers, D. W., Drottar, M., Ptashne, M. & Harrison, S. C. Science 242, 899–907 (1988).
Wolberger, C., Dong, Y., Ptashne, M. & Harrison, S. C. Nature 335, 789–795 (1988).
Jordan, S. R. & Pabo, C. O. Science 242, 893–899 (1988).
Saint-Girons, I., Duchange, N., Cohen, G. N. & Zakin, M. M. J. biol. Chem. 259, 14,282–14,285 (1984).
Saint-Girons, I. et al. J. biol. Chem. 261, 10,936–10,940 (1986).
Phillips, S. E. V. et al. Nature 341, 711–715 (1989).
Rafferty, J. B. et al. J. molec. Biol. 200, 217–219 (1988).
Bricogne, G. Acta Crystallogr. A32, 832–847 (1976).
Wang, B. C. Meth. Enzym. 115, 90–112 (1985).
Hendrickson, W. A. & Konnert, J. H. Biomolecular Structure, Function, Conformation and Evolution Vol. 1 (ed. Srinivasan, R.) 43–57 (Pergamon, Oxford, 1981).
Liljestrand-Golden, C. A. & Johnson, J. R. J. Bact. 157, 413–419 (1984).
Remington, S., Wiegand, G. & Huber, R. J. molec. Biol. 158, 111–152 (1982).
Urbanowski, M. L., Plamann, L. S. & Stauffer, G. V. J. Bact. 169, 126–130 (1987).
McClarin, J. A. et al. Science 234, 1526–1541 (1986).
Suck, D., Lahm, A. & Oefner, C. Nature 332, 464–468 (1988).
Seeman, N. C., Rosenberg, J. M. & Rich, A. Proc. natn. Acad. Sci. U.S.A. 73, 804–808 (1976).
Lee, B. & Richards, F. M. J. molec. Biol. 55, 379–400 (1971).
Zhang, R.-g. et al. Nature 327, 591–597 (1987).
Kumamoto, A. A., Miller, W. G. & Gunsalus, R. P. Genes Dev. 1, 556–564 (1987).
Klig, L. S., Carey, J. & Yanofsky, C. J. J. molec. Biol. 202, 769–777 (1988).
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Rafferty, J., Somers, W., Saint-Girons, I. et al. Three-dimensional crystal structures of Escherichia coli met repressor with and without corepressor. Nature 341, 705–710 (1989). https://doi.org/10.1038/341705a0
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DOI: https://doi.org/10.1038/341705a0
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