The analytical power of high-resolution two-dimensional gel electrophoresis has been coupled with mol-ecular cloning techniques to allow the sequencing of proteins directly from preparative gels.
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References
Kennedy, T.E. et al. Proc. natn. Acad. Sci. U.S.A. 85, 7008–7012 (1988).
Matsudaira, P. J. biol. Chem. 262, 10035–10038 (1987).
Cleveland, D.W., Fischer, S.G., Firschner, M.W. & Laemmli, U.K. J. biol. Chem. 252, 1192–1106 (1970).
Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).
Sweatt, J.D. & Kandel, E.R. (in preparation).
Castellucci, V.F., Kennedy, T.E., Kandel, E.R. & Goelet, P. Neuron 1, 321–328 (1988).
Barzilai, A., Kennedy, T.E., Sweatt, J.D., & Kandel, E.R. (in preparation).
Munro, S. & Pelham, R.B. Cell 46, 291–300 (1986).
Abersold, R.H., Leavitt, J., Saavedra, R.A., Hood, L.E. & Kent, S.B.H. Proc. natn. Acad. Sci. U.S.A. 84, 6970–6974 (1987).
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Kennedy, T., Wager-Smith, K., Barzila, A. et al. Sequencing proteins from acrylamide gels. Nature 336, 499–500 (1988). https://doi.org/10.1038/336499a0
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DOI: https://doi.org/10.1038/336499a0
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