Abstract
Cell-cell adhesion is essential for many immunological functions1–4. The LFA-1 molecule, a member of a superfamily of adhesion molecules, participates in adhesion which is critical to the function of each of the three major subsets of leukocytes: lymphocytes, monocytes and granulocytes5. Putative LFA-1 ligands have been identified functionally in different laboratories using three different monoclonal antibodies that inhibit LFA-1-mediated leukocyte adhesion in particular model systems; however, there may be more than one LFA-1 ligand6–8. We have directly compared the three relevant monoclonal antibodies, and show that each binds to the same molecule, intercellular-adhesion molecule-1 (ICAM-1). Most important, B, T and myeloid cells adhere specifically to purified ICAM-1-coated surfaces; such adhesion has distinctive requirements for Mg2+ and Ca2+. This constitutes biochemical evidence that ICAM-1 functions as a ligand for LFA-1-dependent adhesion by a variety of leukocytes.
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References
Rouse, R. V., Reichert, R. A., Gallatin, W. M., Weissman, I. L. & Butcher, E. C. Am. J. Anat. 170, 391–405 (1984).
Bjerknes, M., Cheng, H. & Ottaway, C. A. Science 231, 402–405 (1986).
Bell, G. I. Immun. Today 4, 237–240 (1983).
Inaba, K. & Steinman, R. M. J. exp. Med. 163, 247–261 (1986).
Springer, T. A., Dustin, M. L., Kishimoto, T. K. & Marlin, S. D. A. Rev. Immun. 5, 223–252 (1987).
Rothlein, R., Dustin, M. L., Marlin, S. D. & Springer, T. A. J. Immun. 137, 1270–1274 (1986).
Makgoba, M. W. et al. Ann. N. Y. Acad. Sci. (in the press).
Makgoba, M. W. et al. Eur. J. Immun. (in the press).
Dustin, M. L., Rothlein, R., Bhan, A. K., Dinarello, C. A. & Springer, T. A. J. Immun. 137, 245–254 (1986).
Clark, E. A., Ledbetter, J. A., Holly, R. C., Dinndorf, P. A. & Shu, G. Hum. Immun. 16, 100–113 (1986).
Patarroyo, M., Clark, E. A., Prieto, J., Kantor, C. & Gahmberg, C. G. FEBS Lett. 210, 127–131 (1987).
Marlin, S. D., Miller, L. M. & Springer, T. A. in Leucocyte typing III, White cell differentiation antigens (eds McMichael, A. J. et al.) 832–833 (Oxford University Press, New York, 1987).
Hogg, N. & Horton, M. A. in Leucocyte Typing III, White Cell Differentiation Antigens (eds McMichael, A. J. et al.) 576–602 (Oxford University Press, Oxford, 1987).
Martz, E. J. Cell Biol. 84, 584–598 (1980).
Shaw, S. et al. Nature 323, 262–264 (1986).
Hynes, R. O. Cell 48, 549–554 (1987).
Lawler, J. & Hynes, R. O. J. Cell Biol. 103, 1635–1648 (1986).
Suzuki, S. et al. Proc. natn. Acad. Sci. U.S.A. 83, 8614–8618 (1986).
Takada, Y., Strominger, J. L. & Hemler, M. E. Proc. natn. Acad. Sci. U.S.A. 84, 3239–3243 (1987).
Law, S. K. A. et al. EMBO J. 6, 915–919 (1987).
Miller, L. J., Wiebe, M. & Springer, T. A. J. Immun. 138, 2381–2383 (1987).
Kishimoto, T. K., O'Connor, K., Lee, A., Roberts, T. M. & Springer, T. A. Cell 48, 681–690 (1987).
Luce, G. G., Gallop, P. M., Sharrow, S. O. & Shaw, S. BioTechniques 3, 270–272 (1985).
Shaw, S. & Luce, G. G. J. Immun. 139, 1037–1045 (1987).
Sanchez-Madrid, F. et al. Proc. natn. Acad. Sci. U.S.A. 79, 7489–7493 (1982).
Barnstable, C. J. et al. Cell 14, 9–20 (1978).
Rubin, L. A., Kurman, C. C., Biddison, W. E., Goldman, N. D. & Nelson, D. L. Hybridoma 4, 91–102 (1985).
Hildreth, J. E. & August, J. T. J. Immun. 134, 3272–3280 (1985).
Schaadt, M., Diehl, V., Stein, H., Fonatsch, C. & Kirchner, H. H. Int. J. Cancer 26, 723–731 (1980).
Quinones, R. R., Lunney, J. & Gress, R. E. Transplantation (in the press).
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Makgoba, M., Sanders, M., Luce, G. et al. ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells. Nature 331, 86–88 (1988). https://doi.org/10.1038/331086a0
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DOI: https://doi.org/10.1038/331086a0
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